Exosomes as Carriers for Notch Molecules

Guya Diletta Marconi; Francesca Diomede; Oriana Trubiani; Cristina Porcheri; Thimios A Mitsiadis

doi:10.1007/978-1-0716-2201-8_16

Exosomes as Carriers for Notch Molecules

Methods Mol Biol. 2022:2472:197-208. doi: 10.1007/978-1-0716-2201-8_16.

Authors

Guya Diletta Marconi^{1

2}, Francesca Diomede³, Oriana Trubiani³, Cristina Porcheri², Thimios A Mitsiadis⁴

Affiliations

¹ Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
² Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, Zurich, Switzerland.
³ Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Chieti, Italy.
⁴ Orofacial Development and Regeneration, Institute of Oral Biology, University of Zurich, Zurich, Switzerland. thimios.mitsiadis@zzm.uzh.ch.

PMID: 35674902
DOI: 10.1007/978-1-0716-2201-8_16

Abstract

Exosomes are extracellular vesicles involved in cell-to-cell communication as well as extrusion of biological material. Using dental pulp stem cells culture as a model, we hereby describe a method for the packaging of Delta-like 4 (DLL4), a representative Notch ligand, into newly generated exosomes. We then provide methods of analysis to confirm the presence of Notch proteins and transcripts internalization and transport via exosomes.

Keywords: Dental pulp stem cells; Exosomes; Extracellular vesicles; Notch pathway; Secretome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Communication
Exosomes* / metabolism
Extracellular Vesicles* / metabolism
Protein Transport
Receptors, Notch / metabolism

Substances

Receptors, Notch