Immunolocalization of Notch Signaling in Mouse Preimplantation Embryos

Methods Mol Biol. 2022:2472:173-185. doi: 10.1007/978-1-0716-2201-8_14.

Abstract

The Notch signaling pathway is an important determinant of cell diversity and identity in most developing embryonic tissues. The pathway components are expressed dynamically, and their function is critical for embryonic survival.This protocol addresses the immunolocalization of Notch pathway components by confocal microscopy.

Keywords: Confocal microscopy; Immunocytochemistry; Mouse; Notch intracellular domain (NICD); Notch signaling; Preimplantation embryo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst* / metabolism
  • Mice
  • Receptors, Notch / metabolism
  • Signal Transduction*

Substances

  • Receptors, Notch