Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing

Dongqing Mo; Jie Shen; Junzheng Zhang

doi:10.1007/978-1-0716-2201-8_4

Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing

Methods Mol Biol. 2022:2472:39-48. doi: 10.1007/978-1-0716-2201-8_4.

Authors

Dongqing Mo¹, Jie Shen¹, Junzheng Zhang²

Affiliations

¹ Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China.
² Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China. zhangjz@cau.edu.cn.

PMID: 35674890
DOI: 10.1007/978-1-0716-2201-8_4

Abstract

Mutations of genes encoding key components of the Notch signaling pathways often result in lethality at early developmental stages, making it difficult to decipher how they regulate the formation of specific cell types or organs. Mosaic analysis using the FLP/FRT system allows investigating the roles of essential genes during wing development in Drosophila melanogaster. This chapter describes the practical methods to isolate Notch signaling regulators by somatic mosaic screen. The fly stocks, cross schemes, and screen parameters are summarized. We also explain how to validate the roles of potential Notch signaling regulators.

Keywords: Drosophila melanogaster; FLP/FRT; Notch signaling; Wing.

MeSH terms

Animals
Drosophila Proteins* / genetics
Drosophila Proteins* / metabolism
Drosophila melanogaster / metabolism
Drosophila* / genetics
Receptors, Notch / genetics
Receptors, Notch / metabolism
Wings, Animal

Substances

Drosophila Proteins
Receptors, Notch