Tracing Lipid Metabolism by Alkyne Lipids and Mass Spectrometry: The State of the Art

Abstract

Lipid tracing studies are a key method to gain a better understanding of the complex metabolic network lipids are involved in. In recent years, alkyne lipid tracers and mass spectrometry have been developed as powerful tools for such studies. This study aims to review the present standing of the underlying technique, highlight major findings the strategy allowed for, summarize its advantages, and discuss some limitations. In addition, an outlook on future developments is given.

Keywords: analog; click; fatty acid; lipidomics; metabolism; probe; tracer; β-oxidation.