Prime editor integrase systems boost targeted DNA insertion and beyond

Ayman Eid; Yiping Qi

doi:10.1016/j.tibtech.2022.05.002

Prime editor integrase systems boost targeted DNA insertion and beyond

Trends Biotechnol. 2022 Aug;40(8):907-909. doi: 10.1016/j.tibtech.2022.05.002. Epub 2022 Jun 3.

Authors

Ayman Eid¹, Yiping Qi²

Affiliations

¹ Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA.
² Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD 20742, USA; Institute for Bioscience and Biotechnology Research, University of Maryland, Rockville, MD 20850, USA. Electronic address: yiping@umd.edu.

PMID: 35667925
DOI: 10.1016/j.tibtech.2022.05.002

Abstract

Previously developed genome engineering tools cannot efficiently direct site-specific long DNA insertion. Built on the prime editing platform, two recent studies have reported integrase-mediated site-specific long DNA integration in the human genome. These prime editor integrase (PEI) systems will unleash many exciting applications in humans, animals, and plants.

Keywords: BxbI; DNA insertion; PASTE; TwinPE; integrase; prime editor.

Publication types

Review
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
CRISPR-Cas Systems
DNA* / genetics
Gene Editing* / methods
Genome, Human
Humans
Integrases* / genetics
Integrases* / metabolism
Plasmids

Substances

DNA
Integrases