Resilience of the wheat root-associated microbiome to the disturbance of phenanthrene

Abstract

The microbial communities are of high importance to the restoration of ecological function and plant health, while little information about the influence of exogenous pollutants on the resilience and temporal dynamics of root microbial communities is available. In this study, a greenhouse experiment was conducted to investigate the effects of exogenous phenanthrene in terms of time and pollution disturbance on the wheat root-associated microbial communities. It was found that a high phenanthrene degradation rate of 86 % was achieved in the rhizosphere of wheat after the first-week planting. Compared to phenanthrene pollution, temporal changes had more significant impacts on the wheat root microbial communities. Obvious change of microbes influenced by PHE had been revealed at the initial three-week planting even most of PHE has been degraded, and the enriched microbes in the rhizosphere were affiliated to Altererythrobacter, Massilia, Mycobacterium, Ramlibacter, Sphingobium, Novosphingobium and Romboutsia. However, at the later stage after four-week incubation, the wheat root-associated microbial communities gradually recovered to the state without pollution. The results of this study were helpful to deepen the understanding of the response of root-associated microbial resilience to the exogenous phenanthrene pollution, and would benefit the stability and balance of agricultural ecology facing exogenous organic pollutants.

Keywords: Microbial resilience; Polycyclic aromatic hydrocarbons; Root microbiome; Temporal variation; Wheat plant.