A description of Cannabinoid levels in Cannabis oil by high-performance liquid chromatography-mass spectrometry in a reference laboratory of North-Italy

Alessandra Manca; Alice Palermiti; Jacopo Mula; Elisa Delia De Vivo; Sandra Zeaiter; Marco Simiele; Amedeo De Nicolò; Marco Cantù; Jessica Cusato; Antonio D'Avolio

doi:10.1016/j.phymed.2022.154218

A description of Cannabinoid levels in Cannabis oil by high-performance liquid chromatography-mass spectrometry in a reference laboratory of North-Italy

Phytomedicine. 2022 Jul 20:102:154218. doi: 10.1016/j.phymed.2022.154218. Epub 2022 May 28.

Authors

Affiliations

¹ Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Medical Sciences, University of Turin, Amedeo di Savoia Hospital, Turin, Italy. Electronic address: alessandra.manca@unito.it.
² Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Medical Sciences, University of Turin, Amedeo di Savoia Hospital, Turin, Italy.
³ Laboratory of Clinical Pharmacology and Pharmacogenetics, Department of Medical Sciences, University of Turin, Amedeo di Savoia Hospital, Turin, Italy; CoQua Lab srl, Italy.
⁴ CoQua Lab srl, Italy.
⁵ Laboratory of Clinical Biochemistry and Pharmacology, Department of Laboratory Medicine EOLAB, Ente Ospedaliero Cantonale, Bellinzona, Switzerland.

PMID: 35665680
DOI: 10.1016/j.phymed.2022.154218

Abstract

Background: Cannabis oils from FM2®, Bedica®, Bediol®, Bedrocan®, Bedrolite® and Pedanios 22/1® are largely used for medical purposes such as spasticity, chronic pain and appetite stimulating. Several studies showed cannabinoids action on CB1 and CB2 receptors reduces the hyperalgesic phase in inflammatory pain, leading to an improvement of conditions. The active compounds of these galenic preparations show a high variability making titration mandatory. For this reason, the exact oil composition knowledge is fundamental for personalizing therapy. This amis at adapting the correct dose to the patient, improving safety and efficacy of the galenic formulation, choosing the best preparation for each patient.

Purpose: The aim of this study was to investigate oil preparations variability among different galenic laboratories in order to highlight the importance of titration activity.

Methods: Cannabis pharmacological active compounds titration has been performed in a large cohort of galenic laboratories in Italy. CBD, CBN, THC, THCA and CBDA quantification was carried out by a previous validated method in UHPLC-MS/MS.

Results: A number of 4318 samples of Cannabis oil from 83 pharmacies between January 2021 and February 2022 were evaluated. All galenic preparation specialities showed statistically significant differences among galenic laboratories (p-value < 0.001). THCA and CBDA concentrations were investigated as percentage of the extration yelds for total THC and CBD: these compounds had different values in the same specialities among distinct galenic laboratories. Moreover, seasonal variability in analytes concentrations was observed.

Conclusion: This study described a wide range of oily samples from a large number of galenic laboratories, compared to published papers. In conclusion, knowledge of the exact oil composition is fundamental in the perspective of personalized therapy. Further studies aiming at the correlation between galenic composition and cannabinoids pharmacokinetics, clinical outcomes and toxic effects could be useful to improve our knowledge.

Keywords: Bediol; Bedrocan; CBD; Medical cannabis; THC.

MeSH terms

Analgesics
Cannabinoids*
Cannabis* / chemistry
Chromatography, High Pressure Liquid / methods
Dronabinol
Humans
Tandem Mass Spectrometry / methods

Substances

Analgesics
Cannabinoids
Dronabinol