Serological characterization of lineage II insect-specific flaviviruses compared with pathogenic mosquito-borne flaviviruses

Koshiro Tabata; Yukari Itakura; Shinsuke Toba; Kentaro Uemura; Mai Kishimoto; Michihito Sasaki; Jessica J Harrison; Akihiko Sato; William W Hall; Roy A Hall; Hirofumi Sawa; Yasuko Orba

doi:10.1016/j.bbrc.2022.05.080

Serological characterization of lineage II insect-specific flaviviruses compared with pathogenic mosquito-borne flaviviruses

Biochem Biophys Res Commun. 2022 Aug 6:616:115-121. doi: 10.1016/j.bbrc.2022.05.080. Epub 2022 May 27.

Authors

Affiliations

¹ Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan.
² Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan; Shionogi & Co., Ltd., Osaka, 541-0045, Japan.
³ Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan; Shionogi & Co., Ltd., Osaka, 541-0045, Japan; Laboratory of Biomolecular Science, Faculty of Pharmaceutical Science, Hokkaido University, Sapporo, 060-0812, Japan.
⁴ Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia, Queensland, Australia.
⁵ International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan; Global Virus Network, Baltimore, MD, 21201, USA; National Virus Reference Laboratory, School of Medicine, University College Dublin, Belfield, Dublin, 4, Ireland.
⁶ Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan; Global Virus Network, Baltimore, MD, 21201, USA; One Health Research Center, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan.
⁷ Division of Molecular Pathobiology, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan; International Collaboration Unit, International Institute for Zoonosis Control, Hokkaido University, Sapporo, Hokkaido, 001-0020, Japan. Electronic address: orbay@czc.hokudai.ac.jp.

PMID: 35665607
DOI: 10.1016/j.bbrc.2022.05.080

Abstract

The genus Flavivirus includes pathogenic tick- and mosquito-borne flaviviruses as well as non-pathogenic insect-specific flaviviruses (ISFVs). Phylogenetic analysis based on whole amino acid sequences has indicated that lineage II ISFVs have similarities to pathogenic flaviviruses. In this study, we used reactive analysis with immune serum against Psorophora flavivirus (PSFV) as a lineage IIa ISFV, and Barkeji virus (BJV) as a lineage IIb ISFV, to evaluate the antigenic similarity among lineage IIa and IIb ISFVs, and pathogenic mosquito-borne flaviviruses (MBFVs). Binding and antibody-dependent enhancement assays showed that anti-PSFV sera had broad cross-reactivity with MBFV antigens, while anti-BJV sera had low cross-reactivity. Both of the lineage II ISFV antisera were rarely observed to neutralize MBFVs. These results suggest that lineage IIa ISFV PSFV has more antigenic similarity to MBFVs than lineage IIb ISFV BJV.

Keywords: Antibody; Antibody-dependent enhancement (ADE); BJV; Cross-reactivity; Lineage II insect-Specific flavivirus; PSFV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Culicidae*
Flavivirus*
Insecta
Phylogeny