Whole-mount immunofluorescent labeling of the adult fly retina

Min-Chun Hsiao; Ting-Yi Huang; Bo-Hua Yu; Tse-Chia Chang; Hui-Yun Chang; Tzu-Kang Sang

doi:10.1016/j.xpro.2022.101430

Whole-mount immunofluorescent labeling of the adult fly retina

STAR Protoc. 2022 May 27;3(2):101430. doi: 10.1016/j.xpro.2022.101430. eCollection 2022 Jun 17.

Authors

Min-Chun Hsiao¹, Ting-Yi Huang¹, Bo-Hua Yu¹, Tse-Chia Chang¹, Hui-Yun Chang², Tzu-Kang Sang¹

Affiliations

¹ Institute of Biotechnology, National Tsing Hua University, Hsinchu 30031, Taiwan.
² Institute of Systems Neuroscience, National Tsing Hua University, Hsinchu 30031, Taiwan.

Abstract

The adult Drosophila compound eye is an ideal in vivo model for studying biological questions. However, light microscopy of this tissue requires cumbersome embedding and sectioning. Here, we document detailed whole-mount procedures for immunolabeling the adult retina, enabling high-quality studies of fluorescent-tagged targets with straightforward preparations. We describe the steps for visualizing the nuclear lamina, membrane-associated protein, and actin-rich rhabdomere, but this robust protocol can apply to other cellular structures and target proteins. For complete details on the use and execution of this protocol, please refer to Chang et al. (2021).

Keywords: Cell Biology; Microscopy; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins
Animals
Drosophila*
Histological Techniques* / methods
Microscopy
Retina / diagnostic imaging

Substances

Actins