Emergence of bla NDM- 1-Carrying Aeromonas caviae K433 Isolated From Patient With Community-Acquired Pneumonia

Xinhua Luo; Kai Mu; Yujie Zhao; Jin Zhang; Ying Qu; Dakang Hu; Yifan Jia; Piaopiao Dai; Jian Weng; Dongguo Wang; Lianhua Yu

doi:10.3389/fmicb.2022.825389

Emergence of bla _{NDM- 1}-Carrying Aeromonas caviae K433 Isolated From Patient With Community-Acquired Pneumonia

Front Microbiol. 2022 May 19:13:825389. doi: 10.3389/fmicb.2022.825389. eCollection 2022.

Authors

Xinhua Luo¹, Kai Mu^{2

3}, Yujie Zhao⁴, Jin Zhang¹, Ying Qu¹, Dakang Hu¹, Yifan Jia¹, Piaopiao Dai¹, Jian Weng⁵, Dongguo Wang⁶, Lianhua Yu¹

Affiliations

¹ Department of Clinical Laboratory Medicine, Taizhou Municipal Hospital Affiliated With Taizhou University, Taizhou, China.
² Beijing Institute of Radiation Medicine, Beijing, China.
³ Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing, China.
⁴ Department of Clinical Laboratory Medicine, Ningbo Medical Center Li Huili Hospital, Ningbo, China.
⁵ Taizhou Center for Disease Control and Prevention, Taizhou, China.
⁶ Department of Central Laboratory, Taizhou Municipal Hospital Affiliated With Taizhou University, Taizhou, China.

Abstract

To demonstrate the detailed genetic characteristics of a bla _NDM-1-carrying multidrug-resistant Aeromonas caviae strain, the complete genome of the A. caviae strain K433 was sequenced by Illumina HiSeq and Oxford nanopore platforms, and mobile genetic elements associated with antibiotic resistance genes were analyzed by a series of bioinformatics methods. A. caviae K433 which was determined to produce class B carbapenemase, was resistant to most antibiotics tested except amikacin. The genome of K433 consisted of a chromosome cK433 (6,482-kb length) and two plasmids: pK433-qnrS (7.212-kb length) and pK433-NDM (200.855-kb length), the last being the first investigated bla _NDM-carrying plasmid from Aeromonas spp. By comparison of the backbone and MDR regions from the plasmids studied, they involved a highly homologous sequence structure. This study provides in-depth genetic insights into the plasmids integrated with bla _NDM-carrying genetic elements from Aeromonas spp.

Keywords: Aeromonas spp.; IMEs; blaNDM; mobile genetic elements; multidrug resistance.