UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutum

Zhongyang Xu; Jiasen He; Muhammad Tehseen Azhar; Zhen Zhang; Senmiao Fan; Xiao Jiang; Tingting Jia; Haihong Shang; Youlu Yuan

doi:10.7717/peerj.13460

UDP-glucose pyrophosphorylase: genome-wide identification, expression and functional analyses in Gossypium hirsutum

PeerJ. 2022 May 30:10:e13460. doi: 10.7717/peerj.13460. eCollection 2022.

Authors

Zhongyang Xu¹, Jiasen He¹, Muhammad Tehseen Azhar², Zhen Zhang³, Senmiao Fan³, Xiao Jiang³, Tingting Jia³, Haihong Shang¹, Youlu Yuan³

Affiliations

¹ Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Zhengzhou, Henan, China.
² Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.
³ State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministryof Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, China.

Abstract

In this study, a total of 66 UDP-glucose pyrophosphorylase (UGP) (EC 2.7.7.9) genes were identified from the genomes of four cotton species, which are the members of Pfam glycosyltransferase family (PF01702) and catalyze the reaction between glucose-1-phosphate and UTP to produce UDPG. The analysis of evolutionary relationship, gene structure, and expression provides the basis for studies on function of UGP genes in cotton. The evolutionary tree and gene structure analysis revealed that the UGP gene family is evolutionarily conserved. Collinearity and Ka/Ks analysis indicated that amplification of UGP genes is due to repetitive crosstalk generating between new family genes, while being under strong selection pressure. The analysis of cis-acting elements exhibited that UGP genes play important role in cotton growth, development, abiotic and hormonal stresses. Six UGP genes that were highly expressed in cotton fiber at 15 DPA were screened by transcriptome data and qRT-PCR analysis. The addition of low concentrations of IAA and GA3 to ovule cultures revealed that energy efficiency promoted the development of ovules and fiber clusters, and qRT-PCR showed that expression of these six UGP genes was differentially increased. These results suggest that the UGP gene may play an important role in fiber development, and provides the opportunity to plant researchers to explore the mechanisms involve in fiber development in cotton.

Keywords: Cotton; Fiber development; UDP-glucose pyrophosphorylase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cotton Fiber
Gene Expression Profiling*
Glucose / metabolism
Gossypium* / genetics
Uridine Diphosphate / metabolism

Substances

Glucose
Uridine Diphosphate

Grants and funding

This work was funded by the Natural Science Foundation of China (NO.3210161266). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.