Surface Functionalization of SBA-15 for Immobilization of Myoglobin

Hengmin Miao; Maosheng Li; Fang Wang; Jiao Li; Ying-Wu Lin; Jiakun Xu

doi:10.3389/fbioe.2022.907855

Surface Functionalization of SBA-15 for Immobilization of Myoglobin

Front Bioeng Biotechnol. 2022 May 19:10:907855. doi: 10.3389/fbioe.2022.907855. eCollection 2022.

Authors

Hengmin Miao^{1

2}, Maosheng Li^{1

3}, Fang Wang¹, Jiao Li², Ying-Wu Lin^{4

5}, Jiakun Xu¹

Affiliations

¹ Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Lab for Marine Drugs and Byproducts of Pilot National Lab for Marine Science and Technology, Qingdao, China.
² School of Materials Science and Engineering, Shandong University of Technology, Zibo, China.
³ School of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
⁴ School of Chemistry and Chemical Engineering, University of South China, Hengyang, China.
⁵ Key Laboratory of Protein Structure and Function of Universities in Hunan Province, University of South China, Hengyang, China.

Abstract

Mesoporous molecular sieve SBA-15 was successfully modified with 3-aminopropyltriethoxysilane (APTES) and 3-glycidyloxypropyltrimethoxysilane (GPTMS). The functionalized SBA-15 were characterized by small-angle X-ray (SAXRD), thermogravimetric analysis (TG), N₂ adsorption, and Fourier transformed infrared spectrum (FT-IR). APTES functionalized SBA-15 (named SBA-15-A) and GPTMS functionalized SBA-15 (named SBA-15-G) were used to immobilize myoglobin (Mb). The loading amounts of Mb by SBA-15-A and SBA-15-G were 511.2 and 547.8 mg/g, respectively, whereas only 359.6 mg/g was achieved by SBA-15. Mb/SBA-15-G and Mb/SBA-15-A demonstrated better reusability than SBA-15, retaining 84.6% and 82.7% of the initial activity after repeated use seven times. The Mb/SBA-15-A and Mb/SBA-15-G also exhibited improved thermal stability and storage stability.

Keywords: SBA-15; biocatalysis; myoglobin; organic functionalization; protein immobilization.