Conditional mutagenesis strategies in zebrafish

Miglė Kalvaitytė; Darius Balciunas

doi:10.1016/j.tig.2022.04.007

Conditional mutagenesis strategies in zebrafish

Trends Genet. 2022 Aug;38(8):856-868. doi: 10.1016/j.tig.2022.04.007. Epub 2022 Jun 2.

Authors

Miglė Kalvaitytė¹, Darius Balciunas²

Affiliations

¹ Life Sciences Center, Vilnius University, Vilnius, Lithuania. Electronic address: migle.kalvaityte@gmc.vu.lt.
² Life Sciences Center, Vilnius University, Vilnius, Lithuania; Department of Biology, Temple University, Philadelphia, PA, USA. Electronic address: darius@temple.edu.

PMID: 35662532
DOI: 10.1016/j.tig.2022.04.007

Abstract

Gene disruption or knockout is an essential tool for elucidating gene function. Conditional knockout methodology was developed to further advance these studies by enabling gene disruption at a predefined time and/or in discrete cells. While the conditional knockout method is widely used in the mouse, technical limitations have stifled direct adoption of this methodology in other animal models including the zebrafish. Recent advances in genome editing have enabled engineering of distinct classes of conditional mutants in zebrafish. To further accelerate the development and application of conditional mutants, we will review diverse methods of conditional knockout engineering and discuss the advantages of different conditional alleles.

Keywords: conditional mutant; genome editing; mutant; recombinase; zebrafish.

Publication types

Review
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Alleles
Animals
Gene Editing* / methods
Mice
Mutagenesis / genetics
Phenotype
Zebrafish* / genetics

Grants and funding

R21 HD103982/HD/NICHD NIH HHS/United States