Frequency of the DI*A, DI*B and Band 3 Memphis polymorphism among distinct groups in Brazil

Alessandra Kaliniczenko; Juliana Oliveira Martins; Bruno Ribeiro Cruz; Akemi Kuroda Chiba; João Paulo Botelho Vieira Filho; José Orlando Bordin

doi:10.1016/j.htct.2022.03.002

Frequency of the DIA, DIB and Band 3 Memphis polymorphism among distinct groups in Brazil

Hematol Transfus Cell Ther. 2023 Jul;45 Suppl 2(Suppl 2):S91-S94. doi: 10.1016/j.htct.2022.03.002. Epub 2022 May 3.

Authors

Alessandra Kaliniczenko¹, Juliana Oliveira Martins², Bruno Ribeiro Cruz², Akemi Kuroda Chiba², João Paulo Botelho Vieira Filho², José Orlando Bordin²

Affiliations

¹ Universidade Federal de São Paulo, Escola Paulista de Medicina, SP, Brazil. Electronic address: ale_kalinic@hotmail.com.
² Universidade Federal de São Paulo, Escola Paulista de Medicina, SP, Brazil.

Abstract

Introduction: The Band 3 is a red blood cell protein that carries the Di^a and Di^b antigens from the Diego blood system. The SLC4A1 gene encodes Band 3; Band 3 Memphis is a polymorphism of normal Band 3 and has two variants, but only the variant II carries the Di^a antigen.

Objectives: Describe the frequencies of the DI*A and DI*B alleles and the Band 3 Memphis among blood donors, sickle cell disease (SCD) patients and Amazonian Indians.

Methods: A total of 427 blood samples were collected and separated into three groups: 206 unrelated blood donors, 90 patients with SCD and 131 Amazonian Indians. We performed DI*A/B, normal Band 3 and Band 3 Memphis genotyping, using the Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP).

Results: The frequency of the DI*A/DI*A genotype was 0.5% in blood donors and it was not found in other groups. The frequency of the DI*A/DI*B was higher in Amazonian Indians (33.6%) and the frequency of the DI*B/DI*B was highest in blood donors (92.2%). All 105 individuals tested were positive for the presence of normal Band 3 and of these individuals, only 5/105 (4.8%) presented the Band 3 Memphis mutation.

Conclusion: We observed a higher frequency of the DI*B allele in blood donors and a low frequency of the DI*A/DI*A genotype in all groups studied. The Band 3 Memphis was found in a higher frequency in the blood donor group. Our findings highlight the importance of analyzing different population groups to gain a better understanding of the genetic association of blood group antigens.

Keywords: Amazonian indians; Blood donors; Diego system; Polymorphism; Red blood cell antigen; Sickle cell disease.