Discovery and genome sequencing of a new virus related to members of the family Tymoviridae, isolated from mosquitoes of the genus Mansonia in Brazil

Keissy Karoline Pinheiro Miranda; Glennda Juscely Pereira Galvão; Pedro Arthur da Silva Araújo; Ana Claudia da Silva Ribeiro; Sandro Patroca da Silva; Poliana da Silva Lemos; Lívia Carício Martins; Márcio Roberto Teixeira Nunes; Pedro Fernando da Costa Vasconcelos; Vânia da Costa Ferreira; Fábio Medeiros da Costa; Rosemary Aparecida Roque; Wanderli Pedro Tadei; Ana Cecilia Ribeiro Cruz; Valéria Lima Carvalho

doi:10.1007/s00705-022-05475-x

Discovery and genome sequencing of a new virus related to members of the family Tymoviridae, isolated from mosquitoes of the genus Mansonia in Brazil

Arch Virol. 2022 Sep;167(9):1889-1892. doi: 10.1007/s00705-022-05475-x. Epub 2022 Jun 5.

Authors

Keissy Karoline Pinheiro Miranda¹, Glennda Juscely Pereira Galvão¹, Pedro Arthur da Silva Araújo¹, Ana Claudia da Silva Ribeiro¹, Sandro Patroca da Silva¹, Poliana da Silva Lemos², Lívia Carício Martins¹, Márcio Roberto Teixeira Nunes², Pedro Fernando da Costa Vasconcelos³, Vânia da Costa Ferreira⁴, Fábio Medeiros da Costa⁵, Rosemary Aparecida Roque⁶, Wanderli Pedro Tadei⁶, Ana Cecilia Ribeiro Cruz⁷, Valéria Lima Carvalho¹

Affiliations

¹ Department of Arbovirology and Hemorrhagic Fevers, Pos Graduate Program in Virology, Evandro Chagas Institute, Ananindeua, PA, 67030-000, Brazil.
² Center for Technological Innovations, Evandro Chagas Institute, Ananindeua, PA, 67030-000, Brazil.
³ Department of Pathology, State University of Pará, Belém, PA, 66087-670, Brazil.
⁴ Energia Sustentável do Brasil, Porto Velho, Rondônia, 76840-000, Brazil.
⁵ Oikos Consultoria e Projetos, 76801-260, Porto Velho, Rondônia, Brazil.
⁶ Malaria and Dengue Laboratory, National Institute of Amazonian Research (INPA), Manaus, Amazonas, 69067-375, Brazil.
⁷ Department of Arbovirology and Hemorrhagic Fevers, Pos Graduate Program in Virology, Evandro Chagas Institute, Ananindeua, PA, 67030-000, Brazil. anacecilia@iec.gov.br.

Abstract

A new virus, named Mutum virus, related to members of the family Tymoviridae, was isolated from mosquitoes (Mansonia spp.) in clone C6/36 cells, and its complete genome was sequenced. Its genome is 6494 nt in size with an organization resembling that of tymovirids. The isolated virus is phylogenetically related to two viruses isolated from Culex spp. mosquitoes: Ek Balam virus, reported in Mexico, and Culex-originated Tymoviridae-like virus, isolated in China. The results of this study suggest that this virus is a new member of the family Tymoviridae.

MeSH terms

Animals
Brazil
Culex*
Culicidae*
Genome, Viral
Malvaceae*
Phylogeny
Tymoviridae* / genetics

Grants and funding

06631-0005/2017/Energia Sustentável do Brasil - ESBR, P&D ANEEL