Potential anti-skin aging effect of a peptide AYAPE isolated from Isochrysis zhanjiangensis on UVB-induced HaCaT cells and H2O2-induced BJ cells

Yuan-Lin He; Liyuan Lin; Haiyan Zheng; Yinhuan Mo; Chunxia Zhou; Shengli Sun; Pengzhi Hong; Zhong-Ji Qian

doi:10.1016/j.jphotobiol.2022.112481

Potential anti-skin aging effect of a peptide AYAPE isolated from Isochrysis zhanjiangensis on UVB-induced HaCaT cells and H₂O₂-induced BJ cells

J Photochem Photobiol B. 2022 Aug:233:112481. doi: 10.1016/j.jphotobiol.2022.112481. Epub 2022 May 26.

Authors

Yuan-Lin He¹, Liyuan Lin¹, Haiyan Zheng¹, Yinhuan Mo¹, Chunxia Zhou², Shengli Sun¹, Pengzhi Hong³, Zhong-Ji Qian⁴

Affiliations

¹ College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524-088, China.
² College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524-088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524-088, China.
³ College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524-088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524-088, China. Electronic address: hongpengzhigdou@163.com.
⁴ College of Food Science and Technology, School of Chemistry and Environment, Shenzhen Institute of Guangdong Ocean University, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Zhanjiang 524-088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524-088, China. Electronic address: zjqian@gdou.edu.cn.

PMID: 35660310
DOI: 10.1016/j.jphotobiol.2022.112481

Abstract

AYAPE (Ala-Tyr-Ala-Pro-Glu) is a pentapeptide isolated from Isochrysis zhanjiangensis, previous studies have proved that this pentapeptide has antioxidant and inflammatory activities. In this study, we determined the anti-skin aging bioactivity of AYAPE with UVB-induced human immortalized keratinocytes (HaCaT) and H₂O₂-induced human skin fibroblasts (BJ cells) as models. The results showed that AYAPE against UVB-induced photoaging on HaCaT cells via alleviating DNA damage, reducing intracellular reactive oxygen (ROS) levels, down regulating phosphorylation of proteins in MAPK/AP-1 signaling pathways. In addition, AYAPE attenuated senescence related effectors expression in H₂O₂-induced BJ cells. Furthermore, p53 showed an important role in regulation effect of AYAPE in both two cells, and AYAPE showed a directly combination with p53 by molecular docking. These results demonstrated that AYAPE is potential to against skin aging by decreasing matrix metalloproteinase-1 (MMP-1) production, inhibiting inflammation and apoptosis, and attenuating fibroblast senescence.

Keywords: Isochrysis zhanjiangensis; MAPK/AP-1; Peptide; Skin aging; p53.

MeSH terms

Fibroblasts / metabolism
HaCaT Cells
Haptophyta* / metabolism
Humans
Hydrogen Peroxide / metabolism
Hydrogen Peroxide / pharmacology
Keratinocytes / metabolism
Molecular Docking Simulation
Peptides / pharmacology
Reactive Oxygen Species / metabolism
Skin Aging*
Tumor Suppressor Protein p53 / metabolism
Ultraviolet Rays

Substances

Peptides
Reactive Oxygen Species
Tumor Suppressor Protein p53
Hydrogen Peroxide