Isolation and molecular characterization of extended spectrum beta lactamase producing Escherichia coli from chicken meat in Pakistan

Zainab Liaqat; Ibrar Khan; Sadiq Azam; Yasir Anwar; Eman Hillal Althubaiti; Lalina Maroof

doi:10.1371/journal.pone.0269194

Isolation and molecular characterization of extended spectrum beta lactamase producing Escherichia coli from chicken meat in Pakistan

PLoS One. 2022 Jun 3;17(6):e0269194. doi: 10.1371/journal.pone.0269194. eCollection 2022.

Authors

Zainab Liaqat¹, Ibrar Khan¹, Sadiq Azam¹, Yasir Anwar², Eman Hillal Althubaiti³, Lalina Maroof⁴

Affiliations

¹ Centre of Biotechnology and Microbiology, University of Peshawar, Peshawar, Pakistan.
² Department of Biological Sciences, Faculty of Science, King Abdul Aziz University, Jeddah, Saudi Arabia.
³ Department of Biotechnology, College of Science, Taif University, Taif, Saudi Arabia.
⁴ City University of Science and Information Technology, Peshawar, Pakistan.

Abstract

The goal of this study was to find E. coli, a prevalent pathogen that causes food-borne illnesses, in chicken samples (n = 500) collected from three districts in KhyberPukhtunkhwa: Mardan, Swabi, and Swat. The E. coli isolates were identified by Gram staining, API strips and Universal Stress Protein. A total of 412 samples tested positive for E. coli and were sensitive to MEM, TZP, and FOS as evidenced by disc diffusion method. The isolates were resistant to TE, NOR, and NA with statistically significant results (P≤0.05). The isolates showed the presence of different antibiotic resistance genes; blaOXA-1, blaCTX-M15, blaTEM-1, QnrS, TetA, AAC, AAD, Sul1 and Sul2. The results revealed mutations in blaOXA-1 gene (H81Q), blaTEM-1 (C108Y, T214A, K284E and P301S), QnrS (H95R) and Sul2 (E66A). The findings of this study may be helpful in better management of E. coli infections by physicians.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Chickens / metabolism
Drug Resistance, Bacterial / genetics
Escherichia coli Infections*
Escherichia coli* / metabolism
Meat
Pakistan
beta-Lactamases / metabolism

Substances

Anti-Bacterial Agents
beta-Lactamases

Grants and funding

The authors are very thankful to the Higher Education Commission, Pakistan for funding the study under grant No. 6678. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.