RNA sequencing analysis between ruptured and un-ruptured brain AVM

Hao Li; Zihan Yan; Ran Huo; Xiaolong Ya; Hongyuan Xu; Zechen Liu; Yuming Jiao; Jiancong Weng; Jie Wang; Shuo Wang; Yong Cao

doi:10.1186/s41016-022-00282-4

RNA sequencing analysis between ruptured and un-ruptured brain AVM

Chin Neurosurg J. 2022 Jun 2;8(1):13. doi: 10.1186/s41016-022-00282-4.

Authors

Hao Li^{1

2}, Zihan Yan^{1

2}, Ran Huo^{1

2}, Xiaolong Ya^{1

2}, Hongyuan Xu^{1

2}, Zechen Liu³, Yuming Jiao^{1

2}, Jiancong Weng^{1

2}, Jie Wang^{1

2}, Shuo Wang^{1

2}, Yong Cao^{4

5

6}

Affiliations

¹ Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 119 South Fourth Ring Road West, Fengtai District, Beijing, 100071, China.
² China National Clinical Research Center for Neurological Diseases, Beijing, China.
³ Department of Biostatistics, Harvard School of Public Health, Boston, USA.
⁴ Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, 119 South Fourth Ring Road West, Fengtai District, Beijing, 100071, China. caoyong@bjtth.org.
⁵ China National Clinical Research Center for Neurological Diseases, Beijing, China. caoyong@bjtth.org.
⁶ Beijing Neurosurgical Institute, Capital Medical University, Beijing, China. caoyong@bjtth.org.

Abstract

Background: A brain arteriovenous malformation (BAVM) is a tangle of abnormal blood vessels connecting the arteries and veins in the brain and is associated with a higher risk for intracerebral hemorrhage (ICH). RNA sequencing technology has been recently used to investigate the mechanism of diseases owing to its ability to identify the gene changes on a transcriptome-wide level. This study aims to gain insights into the potential mechanism involved in BAVM rupture.

Methods: Sixty-five BAVM nidus samples were collected, among which 28 were ruptured and 37 were un-ruptured. Then, next-generation RNA sequencing was performed on all of them to obtain differential expressed genes (DEGs) between the two groups. In addition, bioinformatics analysis was performed to evaluate the involved biological processes and pathways by GO and KEGG analysis. Finally, we performed a univariate Cox regression analysis to obtain the early rupture-prone DEGs.

Results: A total of 951 genes were differentially expressed between the ruptured and un-ruptured BAVM groups, of which 740 genes were upregulated and 211 genes were downregulated in ruptured BAVMs. Then, bioinformatics analysis showed the biological processes and pathways related to the inflammatory processes and extracellular matrix organization were significantly enriched. Meanwhile, some downregulated genes are involved in cell adhesion and genes participating in response to muscle activity and the terms of nervous system development. Finally, one hundred twenty-five genes, many were involved in inflammation, were correlated with the early rupture of BAVMs.

Conclusions: The upregulated genes in the ruptured BAVM group were involved in inflammatory processes and extracellular matrix organization. Some of the downregulated genes participated in cell adhesion and myofibril assembly, indicating the role of enhanced inflammation and reduced inflammation vessel strength in BAVMs rupture.

Keywords: Brain arteriovenous malformation; Cell adhesion; Inflammatory processes; Myofibril assembly; Rupture.

Grants and funding

PXM2019_026280_000002-AVM/the project Genomics Platform Construction for Chinese Major Brain Disease-AVM