Coumarin-based fluorescent probes toward viscosity in mitochondrion/lysosome

Shen Zhang; Yishuo Sun; Wenjie Liu; Wei Feng; Meng Zhang; Zhanxian Li; Mingming Yu

doi:10.1016/j.ab.2022.114752

Coumarin-based fluorescent probes toward viscosity in mitochondrion/lysosome

Anal Biochem. 2022 Sep 1:652:114752. doi: 10.1016/j.ab.2022.114752. Epub 2022 May 30.

Authors

Shen Zhang¹, Yishuo Sun², Wenjie Liu¹, Wei Feng², Meng Zhang³, Zhanxian Li¹, Mingming Yu⁴

Affiliations

¹ Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.
² Department of Chemistry, State Key Laboratory of Molecular Engineering of Polymers, Institutes of Biomedical Sciences & Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, 220 Handan Road, Shanghai, 200433, China.
³ Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China. Electronic address: zhangmeng1991@zzu.edu.cn.
⁴ Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou, 450001, China. Electronic address: yumm@zzu.edu.cn.

PMID: 35654133
DOI: 10.1016/j.ab.2022.114752

Abstract

Viscosity is an important microenvironmental indicator that plays an important role in the process of information transmission in various regions. Herein, two coumarin-based viscosity-sensitive fluorescent probes (CHB, CHN) were synthesized and the photophysical properties of the two probes were studied. The fluorescence quantum yields of CHB and CHN in glycerol can be as high as 25.2% and 18.3% respectively. The two probes can linearly detect viscosity in the viscosity logarithm range of 0.83-2.07, which is not interfered with pH, metal ions, anions and biomolecules. Fluorescent confocal cell experiments show CHB and CHN have good targeting ability to mitochondrion, lysosome, Endoplasmic reticulum and Golgi apparatus, and can be used to detect viscosity in mitochondrion/lysosome.

Keywords: Membrane potential-independent; Mitochondria and lysosomes viscosity detection; Multiple organelle-targeted.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Coumarins / metabolism
Fluorescent Dyes* / chemistry
HeLa Cells
Humans
Lysosomes* / chemistry
Mitochondria / chemistry
Viscosity

Substances

Coumarins
Fluorescent Dyes