Identification of novel biomarkers in chilled and frozen chicken using metabolomics profiling and its application

Abstract

Merchants used frozen chicken to pass it off as chilled chicken, resulting in Economically Motivated Adulteration incidents. Here in this work, firstly, we established OPLS-DA and OPLS-R models based on metabolomics to obtain differential metabolites in chilled and frozen chicken (with different storage times), the PLS-DA model based on above differential compounds could achieve accuracy of 91% (training) and 100% (testing) for the adulteration identification of uncooked chilled and frozen chicken. Secondly, cooking study was carried out to identify the discrepancy of the cooked chilled and frozen chicken. Higher nicotinamide, o-acetyl-l-carnitine, hypoxanthine, and IMP levels indicated better nutrition quality and more desirable flavor in chilled chicken nuggets, while higher bitter and sour peptides in frozen chicken nuggets indicated the loss of freshness.

Keywords: Chilled chicken; Cooked chicken nugget; Frozen chicken; Frozen storage; Metabolomics profiling; Uncooked chicken.