Potassium bromate-induced oxidative stress, genotoxicity and cytotoxicity in the blood and liver cells of mice

Abstract

Potassium bromate (KBrO₃) is an oxidising agent that is extensively used as a food additive, it is also a product of cosmetic and pharmaceutical relevance. The objective of this study was to evaluate the oxidative stress, genotoxicity, and apoptosis induced by KBrO₃ in an experimental animal model. To study the toxic effects and oxidative stress, different doses of KBrO₃ below LD₅₀ (The half maximal lethal dose, 50, 100 and 150 mg/kg body weight) were given intraperitoneally to the mice for multiple time periods (24, 48, and 72 h). The results showed that KBrO₃ significantly induces oxidative damage by increasing the levels of reactive oxygen species (ROS) and lipid peroxidase and depleted the levels of catalase (CAT), superoxide dismutase (SOD) and glutathione (GSH) enzymes in the serum and liver. Moreover, a significant increase of chromosomal aberrations in bone marrow cells and an elevated incidence of micronuclei in the peripheral blood of mice were observed. KBrO₃ induces 3 ´ -OH end double-strand DNA breaks, which was evident in liver sections of the treated mice, and increases the percentage of apoptotic cells, as observed in TUNEL assays and flow cytometry analysis. The present findings indicate that KBrO₃ induces oxidative stress, genotoxicity, and cytotoxicity in a dose- and time-dependent manner in mice.

Keywords: Apoptosis; Cytotoxicity; Genotoxicity; Oxidative stress; Potassium bromate.