A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma

Cheng-Wei Cheng; Wen-Han Feng; Wei-Hua Tang; Hwang-Shang Kou; Chun-Chi Wang; Shou-Mei Wu

doi:10.38212/2224-6614.3391

A three-step stacking capillary electrophoresis of field-amplified sample injection, sweeping, and micellar collapse for determination of dabigatran and its active metabolite in human plasma

J Food Drug Anal. 2022 Mar 15;30(1):88-103. doi: 10.38212/2224-6614.3391.

Authors

Cheng-Wei Cheng¹, Wen-Han Feng², Wei-Hua Tang³, Hwang-Shang Kou¹, Chun-Chi Wang^{1

4

5}, Shou-Mei Wu^{6

7}

Affiliations

¹ School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
² Department of Internal Medicine, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
³ Department of Internal Medicine, Taipei Veterans General Hospital Yuli Branch, Hualian, Taiwan, ROC.
⁴ Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC.
⁵ Drug Development and Value Creation Research Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC.
⁶ Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan, ROC.
⁷ Taiwan Food and Drug Administration, Ministry of Health and Welfare, Taipei, Taiwan, ROC.

Abstract

A three-step stacking capillary electrophoresis (CE) composed of field-amplified sample injection, sweeping, and analyte focusing by micellar collapse (FASI-sweeping-AFMC) was developed to determine dabigatran (D) and its major active metabolite, dabigatran acyl-beta-d-glucuronide (DAG), in human plasma. After optimization and validation, this novel approach was further applied to monitor 5 real samples, and the 25.2-186.8 ng mL^-1 D could be observed among those. Based on these results, the novel CE stacking strategy was successfully applied for the analysis of D and DAG in human plasma and could be served as a tool for clinical assays.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Dabigatran*
Electrophoresis, Capillary / methods
Humans
Micelles*

Substances

Micelles
Dabigatran

Grants and funding

We gratefully acknowledge the support of the Ministry of Science and Technology of Taiwan for grants (MOST 105-2113-M-037-012-MY3 and MOST 108-2113-M-037-018-MY2) funding this work and the assistance from Kaohsiung Medical University Chung-Ho Memorial Hospital.