Direct immersion solid-phase microextraction combined with ambient ionization tandem mass spectrometry to rapidly distinguish pesticides in serum for emergency diagnostics

Hung Su; Hsin-Hua Lin; Lin-Jhen Su; Chia-Chen Lin; Zong-Han Jiang; Shiang-Jin Chen; Jentaie Shiea; Chi-Wei Lee

doi:10.38212/2224-6614.3399

Direct immersion solid-phase microextraction combined with ambient ionization tandem mass spectrometry to rapidly distinguish pesticides in serum for emergency diagnostics

J Food Drug Anal. 2022 Mar 15;30(1):26-37. doi: 10.38212/2224-6614.3399.

Authors

Hung Su¹, Hsin-Hua Lin², Lin-Jhen Su², Chia-Chen Lin², Zong-Han Jiang², Shiang-Jin Chen³, Jentaie Shiea^{1

4

5}, Chi-Wei Lee²

Affiliations

¹ Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan.
² Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan.
³ Department of Emergency Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
⁴ Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan.
⁵ Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Abstract

Despite the fact that carbamates and organophosphates cause acute poisoning via different mechanisms and require disparate management, they are indistinguishable by current clinical assays. Herein, direct immersion solid-phase microextraction (DI-SPME) plus thermal desorption-electrospray ionization tandem mass spectrometry (TD-ESI/MS/MS) was developed to discern them. Both pesticides spiked in human serum were extracted by SPME and analyzed by TD-ESI/MS/MS. This is a promising emergency care platform as rapid analyses could be done in tiny sample volumes with satisfactory recovery (89.46%-116.32%), precision (covariance <20%), sensitivity (LOD <0.1 μg/mL), turnaround time (<5 minutes), and linearity (R² = 0.9827-0.9992) within 0.1-100 μg/mL.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Immersion
Pesticides* / analysis
Solid Phase Microextraction* / methods
Tandem Mass Spectrometry / methods

Substances

Pesticides

Grants and funding

This work was partially supported by the NSYSUKMU Joint Research Project (NSYSUKMU 109-I009), the Ministry of Science and Technology of Taiwan (108-2320-B-110-002-) and the Research Center for Environmental Medicine (Kaohsiung Medical University, Kaohsiung, Taiwan) from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan and by the Kaohsiung Medical University Research Center Grant (KMU-TC109A01).