Associations of Blautia Genus With Early-Life Events and Later Phenotype in the NutriHS

Renata G Borges de Oliveira Nascimento Freitas; Ana Carolina J Vasques; Gabriel da Rocha Fernandes; Francieli B Ribeiro; Isabela Solar; Marina G Barbosa; Bianca de Almeida- Pititto; Bruno Geloneze; Sandra Roberta G Ferreira

doi:10.3389/fcimb.2022.838750

Associations of Blautia Genus With Early-Life Events and Later Phenotype in the NutriHS

Front Cell Infect Microbiol. 2022 May 12:12:838750. doi: 10.3389/fcimb.2022.838750. eCollection 2022.

Authors

Affiliations

¹ Department of Epidemiology, School of Public Health, University of São Paulo, São Paulo, Brazil.
² Laboratory of Investigation in Metabolism and Diabetes, Gastrocentro, School of Medical Sciences, University of Campinas, Campinas, Brazil.
³ School of Applied Sciences, University of Campinas, Campinas, Brazil.
⁴ Oswaldo Cruz Foundation, Belo Horizonte, Brazil.
⁵ Department of Preventive Medicine, Federal University of São Paulo, São Paulo, Brazil.
⁶ Obesity and Comorbidities Research Center, University of Campinas, Campinas, Brazil.

Abstract

Introduction: Early-life events are associated with the risk of obesity and comorbidities later in life. The gut microbiota-whose composition is influenced by genetics and environmental factors-could be involved. Since the microbiota affects metabolism and fat storage, early-life insults could contribute to the occurrence of obesity driven, in part, by microbiota composition. We examined associations of gut bacteria with early-life events, nutritional status, and body composition in the Nutritionist's Health Study (NutriHS).

Methods: A cross-sectional study of 114 female participants examining early-life data, body composition, and biological samples was conducted. Fecal microbiota structure was determined targeting the V4 region of the 16S rRNA gene. Principal coordinates analysis (PCoA) and permutational multivariate analysis of variance (PERMANOVA) were used to test the impact of variables on microbial diversity. Profiles were identified using the Jensen-Shannon divergence matrix and Calinski-Harabasz index. Differential abundance between the categories of exclusive breastfeeding duration and nutritional status was tested using DESeq2.

Results: In the sample [median age 28 years and body mass index (BMI) 24.5 kg/m²], 2 microbiota profiles driven by the Blautia or Prevotella genus were identified. An estimated 9.1% of the variation was explained by the profiles (p < 0.001), 2.1% by nutritional status (p = 0.004), and 1.8% by exclusive breastfeeding (p = 0.012). The proportion of participants with BMI <25 kg/m² and who were breastfed for at least 6 months was higher in the Blautia profile (p < 0.05).

Conclusion: Findings in a Blautia-driven profile of healthy women reinforce that early-life events play a role in defining gut microbiota composition, confirming the importance of exclusive breastfeeding for infant gut colonization in establishing a protective profile against adiposity-related outcomes in adulthood.

Keywords: DOHAD; breastfeeding; early-life events; gut microbiota; nutritional status.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Clostridiales* / genetics
Cross-Sectional Studies
Female
Humans
Obesity* / genetics
Phenotype
RNA, Ribosomal, 16S / genetics

Substances

RNA, Ribosomal, 16S