The Effect of Yiqi Huoxue Tongluo Decoction on Spinal Cord Microglia Activation and ASK1-MKK3-p38 Signal Pathway in Rats with Diabetic Neuropathic Pain

Fanjing Wang; Heyong Tang; Junlong Ma; Lianzhi Cheng; Yixuan Lin; Jindong Zhao; Guoming Shen; Zhaohui Fang; Aijuan Jiang

doi:10.1155/2022/2408265

The Effect of Yiqi Huoxue Tongluo Decoction on Spinal Cord Microglia Activation and ASK1-MKK3-p38 Signal Pathway in Rats with Diabetic Neuropathic Pain

Evid Based Complement Alternat Med. 2022 May 19:2022:2408265. doi: 10.1155/2022/2408265. eCollection 2022.

Authors

Fanjing Wang¹, Heyong Tang², Junlong Ma³, Lianzhi Cheng², Yixuan Lin¹, Jindong Zhao¹, Guoming Shen², Zhaohui Fang^{1

4

5}, Aijuan Jiang²

Affiliations

¹ Department of Endocrine, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China.
² School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei 230012, China.
³ Anhui Jing'an Hospital of Integrated Traditional Chinese and Western Medicine, Hefei 230011, China.
⁴ Diabetes Institute, Anhui Academy of Chinese Medicine, Hefei 230038, China.
⁵ Key Laboratory of Xin'an Medicine, Ministry of Education, Hefei 230038, China.

Abstract

Diabetic neuropathic pain (DNP) is one of the most common chronic peripheral neuropathies in diabetes mellitus (DM). Objective. To observe the underlying mechanism of the effects of Yiqi Huoxue Tongluo Decoction (YQHX) on DNP rats. Methods. SD rats were intraperitoneally injected with 35 mg/kg streptozotocin (STZ) to prepare DNP models and were treated with YQHX for 8 weeks. Results. Studies have shown that the drug restores some levels of MWT, TWL, and MNCV, downregulates the levels of inflammatory factors IL-6, IL-1β, and TNF-α, downregulates the levels of ASK1-MKK3-p38, and weakens the level of OX42 activation. Conclusion. Yiqi Huoxue Tongluo Decoction can relieve DNP by affecting the activity of spinal cord microglia and the ASK1-MKK3-p38 signaling pathway, thereby reducing the central sensitization caused by the inflammatory response of DNP rats.