Cucurbitaceae phloem exudate lectins: Purification, molecular characterization and carbohydrate binding characteristics

Abstract

Much of the plant lectin research was focused on these proteins from seeds, whereas lectins from other plant tissues have been less investigated. Although presence of lectins in the phloem exudate of Cucurbitaceae species was reported over 40 years ago, only a few proteins from this family have been purified and characterized with respect to ligand binding properties, primary and secondary structures, while no 3D structure of a member of this family is known so far. Unlike lectins from other plant families and sources (e.g., seeds and tubers), which exhibit specificity towards different carbohydrate structures, all the Cucurbitaceae phloem exudate lectins characterized so far have been shown to recognize only chitooligosaccharides or glycans containing chitooligosaccharides. Interestingly, some of these proteins also bind various types of RNAs, suggesting that they may also play a role in the transport of RNA information molecules in the phloem. The present review gives an overview of the current knowledge of Cucurbitaceae phloem exudate lectins with regard to their purification, determination of primary and secondary structures, elucidation of thermodynamics and kinetics of carbohydrate binding and computational modeling to get information on their 3D structures. Finally, future perspectives of research on this important class of proteins are considered.

Keywords: Anti-pathogenic activity; Chitooligosaccharide-specific lectin; Computational modeling; Fluorescence spectroscopy; Isothermal titration calorimetry; Phloem protein 2; RNA binding; Wound sealing.