A colorimetric smartphone-based platform for pesticides detection using Fe-N/C single-atom nanozyme as oxidase mimetics

Jia Ge; Like Yang; Zhaohui Li; Yi Wan; Dongsheng Mao; Ruijie Deng; Qi Zhou; Yu Yang; Weihong Tan

doi:10.1016/j.jhazmat.2022.129199

A colorimetric smartphone-based platform for pesticides detection using Fe-N/C single-atom nanozyme as oxidase mimetics

J Hazard Mater. 2022 Aug 15:436:129199. doi: 10.1016/j.jhazmat.2022.129199. Epub 2022 May 21.

Authors

Jia Ge¹, Like Yang¹, Zhaohui Li¹, Yi Wan², Dongsheng Mao³, Ruijie Deng⁴, Qi Zhou⁵, Yu Yang⁶, Weihong Tan⁷

Affiliations

¹ College of Chemistry, Zhengzhou University, Zhengzhou 450001, China.
² Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and Pharmaceutical Sciences, Marine College, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China. Electronic address: 993602@hainu.edu.cn.
³ Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
⁴ China College of Biomass Science and Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China.
⁵ College of Chemistry and Chemical Engineering, Anhui University, Hefei 230601, China. Electronic address: roundzking@163.com.
⁶ Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. Electronic address: yuyang@shsmu.edu.cn.
⁷ Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China; The Cancer Hospital of the University of Chinese Academy of Sciences (Zhejiang Cancer Hospital), Institute of Basic Medicine and Cancer (IBMC), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. Electronic address: tan@hnu.edu.cn.

PMID: 35643002
DOI: 10.1016/j.jhazmat.2022.129199

Abstract

In this study, a novel highly sensitive colorimetric platform has been designed for malathion assay based on Fe-N/C SAzyme. The as-synthesized SAzyme can directly oxidize 3,3´,5,5´-tetramethylbenzidine (TMB) to generate blue colored oxidized TMB. L-ascorbic acid-2-phosphate (AA2P), a substrate of acid phosphatase (ACP), could be hydrolyzed to AA, thereafter inhibit the oxidization reaction of TMB, leading to a conspicuous blue color fading. With the addition of malathion hindered the ACP activity and limited the AA production, resulting in the recovery of the catalytic activity of single-atom nanozyme. Under optimized operational conditions, a novel colorimetric assay has been designed for malathion detection with LOD of 0.42 nM. Besides, quantification of malathion in environmental and food samples was achieved based on the proposed strategy. In addition, the successfully integrated paper/smartphone sensor provided sensitive, and rapid, reliable detection of malathion with a LOD of 1 nM.

Keywords: Acid phosphatase; Colorimetric; Fe-N/C SAzyme; Malathion; Smartphone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Colorimetry* / methods
Limit of Detection
Malathion
Oxidoreductases
Pesticides*
Smartphone

Substances

Pesticides
Oxidoreductases
Malathion