Quick and ultra-sensitive digital assay of influenza virus using sub-picoliter microwells

Hiroki Ashiba; Masato Yasuura; Takashi Fukuda; Ken Hatano; Makoto Fujimaki

doi:10.1016/j.aca.2022.339926

Quick and ultra-sensitive digital assay of influenza virus using sub-picoliter microwells

Anal Chim Acta. 2022 Jun 22:1213:339926. doi: 10.1016/j.aca.2022.339926. Epub 2022 May 12.

Authors

Hiroki Ashiba¹, Masato Yasuura², Takashi Fukuda², Ken Hatano³, Makoto Fujimaki²

Affiliations

¹ Sensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan. Electronic address: h.ashiba@aist.go.jp.
² Sensing System Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
³ Graduate School of Science and Engineering, Saitama University, Saitama, Japan.

PMID: 35641063
DOI: 10.1016/j.aca.2022.339926

Abstract

Quick and sensitive virus detection methods have been developed using sub-picoliter microwells. One method uses an aggregation-induced emission (AIE) reagent, and the other uses an enzymatic reaction supported with immunomagnetic beads at a high concentration of 10⁸ particles/mL. Examination of influenza A virus detection using the AIE reagent exhibited a detection limit of 3 × 10⁵ copies/mL, which was achievable within 1 min of the total measurement time. The high-concentration immunomagnetic beads method exhibited a detection limit as low as 1 × 10² copies/mL. The developed methods are effective and practical tools for ultrafast and ultrasensitive virus detection.

Keywords: Aggregation-induced emission; Digital assay; Fluorescent substance; Magnetic beads; Microwell array; Virus detection.

MeSH terms

Biological Assay
Immunomagnetic Separation
Influenza A virus*
Orthomyxoviridae*