An ultrasensitive biosensor for virulence ompA gene of Cronobacter sakazakii based on boron doped carbon quantum dots-AuNPs nanozyme and exonuclease III-assisted target-recycling strategy

Xiaodong Cao; Minli Liu; Jie Lu; Hongge Lv; Jinzhe Han; Shudong He; Yongkang Ye; Xiangyang Chen; Zhaojun Wei; Haisong Zheng

doi:10.1016/j.foodchem.2022.133268

An ultrasensitive biosensor for virulence ompA gene of Cronobacter sakazakii based on boron doped carbon quantum dots-AuNPs nanozyme and exonuclease III-assisted target-recycling strategy

Food Chem. 2022 Oct 15:391:133268. doi: 10.1016/j.foodchem.2022.133268. Epub 2022 May 20.

Authors

Xiaodong Cao¹, Minli Liu¹, Jie Lu², Hongge Lv², Jinzhe Han², Shudong He¹, Yongkang Ye³, Xiangyang Chen⁴, Zhaojun Wei⁵, Haisong Zheng⁶

Affiliations

¹ School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
² School of Food Science and Biological Engineering, Hefei University of Technology-Xuancheng Campus, Xuancheng 242000, China.
³ School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Collaborative Innovation Center for Food Production and Safety, School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China. Electronic address: yongkang.ye@hfut.edu.cn.
⁴ School of Life and Environmental Sciences, Huangshan University, Huangshan 245041, China.
⁵ School of Food Science and Biological Engineering, Hefei University of Technology, Hefei 230009, China; Collaborative Innovation Center for Food Production and Safety, School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China. Electronic address: zjwei@hfut.edu.cn.
⁶ Technology Center of Hefei Customs District, Hefei 230032, Anhui, China. Electronic address: 32789064@qq.com.

PMID: 35640339
DOI: 10.1016/j.foodchem.2022.133268

Abstract

This work presented an electrochemical biosensor for the detection of virulence outer membrane protein A (ompA) gene of Cronobacter sakazakii (C. sakazakii), which was based on the mimic peroxidase activity of boron doped quantum dots-Au nanoparticles (BQDs-AuNPs) and a signal amplification strategy of exonuclease III (Exo III)-assisted target-recycling (EATR). The electrochemical signal was come from the electrochemical reduction of H₂O₂ by BQDs-AuNPs nanozyme. Due to the enhanced peroxidase-mimic electrocatalytic efficiency of BQDs-AuNPs and the EATR strategy, the biosensor showed a broad linear range (1.0 × 10^-15 - 1.0 × 10^-8 mol L^-1) and a low limit of detection (LOD, 4.0 × 10^-17 mol L^-1). The constructed biosensor could also be applied in direct detection of extracted DNA from C. sakazakii. A good linear relationship (7.8 - 7.8 × 10⁶ CFU mL^-1) between the logarithm concentration of C. sakazakii and electrochemical signal was obtained with a LOD of 2.6 CFU mL^-1. The biosensor was applied in the detection of impA gene segments in contaminated infant formula with recoveries ranged in 83.4 - 108.2%.

Keywords: Boron doped carbon quantum dots-gold nanoparticles; Cronobacter sakazakii; Electrochemical biosensor; Exonuclease III-assisted target-recycling; Mimic peroxidase activity; OmpA gene.

MeSH terms

Bacterial Outer Membrane Proteins
Biosensing Techniques*
Boron
Carbon
Cronobacter sakazakii*
Electrochemical Techniques
Exodeoxyribonucleases
Gold
Humans
Hydrogen Peroxide
Infant
Metal Nanoparticles*
Peroxidases
Quantum Dots*
Virulence

Substances

Bacterial Outer Membrane Proteins
OMPA outer membrane proteins
Carbon
Gold
Hydrogen Peroxide
Peroxidases
Exodeoxyribonucleases
exodeoxyribonuclease III
Boron