Low-frequency ultrasound and nitrogen limitation induced enhancement in biomass production and lipid accumulation of Tetradesmus obliquus FACHB-12

Qun Wei; Jinjie Yao; Ruge Chen; Shangru Yang; Yonghe Tang; Xiangmeng Ma

doi:10.1016/j.biortech.2022.127387

Low-frequency ultrasound and nitrogen limitation induced enhancement in biomass production and lipid accumulation of Tetradesmus obliquus FACHB-12

Bioresour Technol. 2022 Aug:358:127387. doi: 10.1016/j.biortech.2022.127387. Epub 2022 May 27.

Authors

Qun Wei¹, Jinjie Yao¹, Ruge Chen¹, Shangru Yang¹, Yonghe Tang², Xiangmeng Ma³

Affiliations

¹ School of Resources, Environment and Materials, Guangxi University, Nanning, Guangxi 530004, PR China.
² Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China; MOE Key Laboratory of New Processing Technology for Nonferrous Metals and Materials, Guangxi Key Laboratory of Processing for Non-ferrous Metals and Featured Materials, Guangxi University, Nanning 530004, PR China.
³ School of Resources, Environment and Materials, Guangxi University, Nanning, Guangxi 530004, PR China; Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China. Electronic address: maxiangmeng@gxu.edu.cn.

PMID: 35636673
DOI: 10.1016/j.biortech.2022.127387

Abstract

The two-stage cultivation strategy was optimized in this study to simultaneously promote the growth and lipid accumulation of Tetradesmus obliquus. Results showed that the optimal dual-stress conditions were nitrogen concentration at 25 mg N·L^-1 and low-frequency ultrasound at 200 Watt, 1 min, and 8 h interval. The biomass and lipid content of Tetradesmus obliquus were increased by 32.1% and 44.5%, respectively, comparing to the control, and the lipid productivity reached 86.97 mg^-1·L^-1·d^-1 at the end of the cultivation period. The protein and photosynthetic pigment contents of microalgae decreased by 22.4% and 14.0% under dual stress comparing to the control environment. In addition, dual stress cultivation of microalgae presented higher level of antioxidant capacity to balance to oxidation level in microalgal cells. This study provides a new insight for microalgae growth and lipid accumulation with dual stress stimulation.

Keywords: CO(NH(2))(2); Dual stress; Lipid accumulation; Nitrogen limitation; Two-stage cultivation strategy; Ultrasonic.

MeSH terms

Biomass
Chlorophyceae* / metabolism
Lipids
Microalgae* / metabolism
Nitrogen / metabolism

Substances

Lipids
Nitrogen