Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers

Kiersten D Lenz; Katja E Klosterman; Harshini Mukundan; Jessica Z Kubicek-Sutherland

doi:10.1016/j.ab.2022.114747

Lipoprotein capture ELISA method for the sensitive detection of amphiphilic biomarkers

Anal Biochem. 2022 Sep 1:652:114747. doi: 10.1016/j.ab.2022.114747. Epub 2022 May 27.

Authors

Kiersten D Lenz¹, Katja E Klosterman¹, Harshini Mukundan¹, Jessica Z Kubicek-Sutherland²

Affiliations

¹ Physical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory, Los Alamos, NM, United States.
² Physical Chemistry and Applied Spectroscopy, Chemistry Division, Los Alamos National Laboratory, Los Alamos, NM, United States. Electronic address: jzk@lanl.gov.

PMID: 35636461
DOI: 10.1016/j.ab.2022.114747

Abstract

Enzyme-linked immunosorbent assays (ELISAs) are widely employed for the detection of protein targets due to their ease of use, sensitivity, and potential for high-throughput analyses. However, the use of ELISAs to detect non-protein targets such as lipids and amphiphiles is complicated by the physical properties of these molecules, which affects their association with functional surfaces and recognition ligands. Here, we developed a unique lipoprotein capture ELISA in which the natural association between lipoproteins and amphiphilic molecules facilitates detection of the target biomarker in a physiologically relevant conformation. An assay to detect the glycolipid lipoarabinomannan (LAM), a cell membrane component and virulence factor associated with Mycobacterial infections, was developed as a proof of concept.

Keywords: Amphiphile; Detection; Enzyme-linked immunosorbent assay; Lipoarabinomannan; Lipoprotein; Nanodisc.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Biomarkers
Enzyme-Linked Immunosorbent Assay / methods
Lipopolysaccharides*
Lipoproteins*
Sensitivity and Specificity

Substances

Biomarkers
Lipopolysaccharides
Lipoproteins