Research over the last two decades has revealed that bacterial genomes are highly organized and that bacteria have sophisticated mechanisms in place to ensure their correct replication and segregation into progeny cells. Here we discuss techniques that can be used with live bacterial cells to analyze DNA replisome dynamics, double-strand chromosome breaks, and restart of repaired replication forks.
Keywords: DNA damage; DNA replication; Double-strand DNA breaks; Replication restart; Replisome; SOS response.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.