Measuring Nuclear Mechanics with Atomic Force Microscopy

Ália Dos Santos; Florian Rehfeldt; Christopher P Toseland

doi:10.1007/978-1-0716-2221-6_13

Measuring Nuclear Mechanics with Atomic Force Microscopy

Methods Mol Biol. 2022:2476:171-181. doi: 10.1007/978-1-0716-2221-6_13.

Authors

Ália Dos Santos¹, Florian Rehfeldt², Christopher P Toseland³

Affiliations

¹ Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK.
² University of Bayreuth, Experimental Physics 1, Bayreuth, Germany. florian.rehfeldt@uni-bayreuth.de.
³ Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK. c.toseland@sheffield.ac.uk.

PMID: 35635704
DOI: 10.1007/978-1-0716-2221-6_13

Abstract

Atomic force microscopy is an ideal tool to map topography and mechanical properties of materials on the micro- and nanoscale. Here, we describe its application to measure and analyze the mechanics, in particular the effective Young's elastic modulus E^* of the mammalian nucleus in live cells. We present three approaches which enable the mechanics to be probed under varying conditions. This includes fully adhered cells, initially adhered cells which lack an established cytoskeleton, and purified nuclei to study their isolated response.

Keywords: Atomic force microscopy; Mechanics; Mechanobiology; Nucleus; Young’s modulus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Nucleus*
Elastic Modulus
Mammals*
Microscopy, Atomic Force

Abstract

Publication types

MeSH terms

Grants and funding