Escherichia coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis

Abstract

Flow cytometry is a high-throughput technique that analyzes individual particles as they pass through a laser beam. These particles can be individual cells and by detecting cell-scattered light their number and relative size can be measured as they pass through the beam. Labeling of molecules, usually via a fluorescent reporter, allows the amount of these molecules per cell to be quantified. DNA content can be estimated using this approach and here we describe how flow cytometry can be used to assess the DNA content of Escherichia coli cells.

Keywords: Chromosome; DnaA; Escherichia coli; Flow cytometry; Genome content; Genome copy number; Replication.