Incorporation of reporter genes into mitochondrial DNA in budding yeast

Magdalena Rzepka; Tamara Suhm; Martin Ott

doi:10.1016/j.xpro.2022.101359

Incorporation of reporter genes into mitochondrial DNA in budding yeast

STAR Protoc. 2022 May 25;3(2):101359. doi: 10.1016/j.xpro.2022.101359. eCollection 2022 Jun 17.

Authors

Magdalena Rzepka^{1

2}, Tamara Suhm¹, Martin Ott^{1

2}

Affiliations

¹ Department of Biochemistry and Biophysics, Stockholm University, 10691 Stockholm, Sweden.
² Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 41390 Gothenburg, Sweden.

Abstract

Many aspects of mitochondrial gene expression are still unknown, which can be attributed to limitations in molecular tools. Here, we present a protocol to introduce reporter genes into the mitochondrial genome of budding yeast, Saccharomyces cerevisiae. Mitochondrially encoded reporter constructs can be used to interrogate various aspects of mitochondrial gene expression. The power of this technique is exemplified by a mitochondrially encoded nanoluciferase, which allows to monitor levels of mitochondrial translation under a variety of growth conditions.

Keywords: Cell Biology; Genetics; Model Organisms; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Mitochondrial* / genetics
Genes, Reporter*
Mitochondria / genetics
Protein Biosynthesis
Saccharomyces cerevisiae* / genetics

Substances

DNA, Mitochondrial