Effect of particulate matters on inactivation of bacteriophage MS2 under irradiation above 320 nm

Abstract

The inactivation of bacteriophage MS2 under irradiation above 320 nm was investigated, focusing on different solution pH, ionic strength, and Suwannee River natural organic matter (SRNOM) concentrations when solutions contained organic or inorganic particle matters. Inorganic and organic particles were modeled using kaolinite (KAO) and Microcystis aeruginosa (MA), respectively. The results showed that the two types of particles influenced on MS2 inactivation under different conditions. The lower pH contributed to the greater MS2 aggregation within pH range of 3.0 to 8.0, leading to an increasing inactivation rate. The presence of KAO induced reactive oxygen species (ROS) under the action of irradiation above 320 nm, which promoted the inactivation of MS2. By comparison, the [Formula: see text] produced by MA after irradiation promoted the inactivation at pH < 6, whereas when the pH is ≥ 6, the inactivation effect of MS2 was lower than that of particle-free solution because MS2 was no longer aggregated and MA has a shading effect. In the presence of Na⁺ or Ca²⁺ cation, irradiation above 320 nm could not effectively inactivate the MS2 under particle-free solution. By comparison, KAO increased the inactivation efficiency as a photosensitizer. With the increase of Ca²⁺ concentration, MS2 was more easily adsorbed to MA than aggregation. Until Ca²⁺ concentration reached 20 mM, the inactivation effect in MA solution was enhanced. In the presence of SRNOM, the inactivation effect increased with the increase of SRNOM concentration. When the SRNOM was 20 mM, the inactivation increased in the particle-free solution due to the greater production of [Formula: see text]. Compared with the particle-free solution, the KAO and MA inactivation efficiency was lower.

Keywords: Inactivation; Irradiation above 320 nm; Kaolinite; MS2; Microcystis aeruginosa.