Effects of sinoaortic denervation on hemodynamic perturbations of prolonged paradoxical sleep deprivation and rapid cold stress in rats

Yia-Ping Liu; Chen-Cheng Lin; Yu-Chieh Lin; Shih-Hung Tsai; Che-Se Tung

doi:10.31083/j.jin2103075

Effects of sinoaortic denervation on hemodynamic perturbations of prolonged paradoxical sleep deprivation and rapid cold stress in rats

J Integr Neurosci. 2022 Apr 6;21(3):75. doi: 10.31083/j.jin2103075.

Authors

Yia-Ping Liu^{1

2}, Chen-Cheng Lin², Yu-Chieh Lin³, Shih-Hung Tsai⁴, Che-Se Tung^{2

3}

Affiliations

¹ Department of Psychiatry, Cheng Hsin General Hospital, 11280 Taipei, Taiwan.
² Department of Physiology, National Defense Medical Center, 11490 Taipei, Taiwan.
³ Division of Medical Research & Education, Cheng Hsin General Hospital, 11280 Taipei, Taiwan.
⁴ Department of Emergency Medicine, Tri-service General Hospital, National Defense Medical Center, 11490 Taipei, Taiwan.

PMID: 35633156
DOI: 10.31083/j.jin2103075

Abstract

Background: Sleep disturbances and aversive cold stress (CS) are cardiovascular risk factors. This study investigates how homeostatic control autonomic baroreflex influences the hemodynamic perturbations evoked by paradoxical sleep deprivation (PSD) and CS.

Methods: Conscious adult male rats were randomly divided into four groups (Sham/CON [control], Sham/PSD, sinoaortic denervation [SAD]/CON, and SAD/PSD). Spectral analysis and SAD were employed to evaluate the effects of a 72-hr PSD with 10-min CS on blood pressure variability and heart rate variability (BPV and HRV) at total power (TP) and three frequency power densities, very-low-frequency (VLF), low frequency (LF), and high frequency (HF).

Results: Key findings showed: (I) Compared with the control sham surgery (Sham/CON), in the natural baseline (PreCS) trial, SAD surgery (SAD/CON) causes high systolic blood pressure (SBP), heart rate (HR), increases LFBPV (low-frequency power of BPV), LF/HFHRV (the ratio LF/HF of HRV), and TPBPV (the total power of BPV), but decreases HFHRV (high-frequency power of HRV) and VLFHRV (very-low-frequency power of HRV) than the Sham/CON does. In the CS trial, SAD/CON increases the CS-induced pressor, increases the CS-elicited spectral density, LF/HFHRV, but decreases HFBPV than the Sham/CON does. (II) Compared with SAD/CON and Sham/PSD (PSD under sham surgery), in both PreCS and CS trials, SAD/PSD (PSD under SAD) causes high SBP and HR than both SAD/CON and Sham/PSD their SBP and HR. In PreCS, SAD-PSD also changes the spectral density, including increasing Sham-PSD's LFBPV, LF/HFHRV, VLFBPV, and TPBPV but decreasing Sham-PSD's VLFHRV and TPHRV. However, in CS, SAD-PSD changes the CS-elicited spectral density, including increasing Sham-PSD's VLFBPV, LF/HFHRV, and TPHRV but decreasing Sham-PSD's HFBPV and LFBPV.

Conclusion: The results suggest baroreflex combined with other reflex pathways, such as inhibitory renorenal reflex, modulates the vascular and cardiorespiratory responses to PSD under PreCS and subsequent CS trials.

Keywords: Blood pressure variability; Cold stress; Heart rate variability; Paradoxical sleep deprivation; Sinoaortic denervation; Spectral analysis.

MeSH terms

Animals
Cold-Shock Response*
Denervation
Heart Rate / physiology
Hemodynamics / physiology
Male
Rats
Sleep, REM*