Molecular Analysis of Caprine Enterovirus Circulating in China during 2016-2021: Evolutionary Significance

Viruses. 2022 May 15;14(5):1051. doi: 10.3390/v14051051.

Abstract

Here, we report the characterization of 13 novel caprine/ovine enterovirus strains isolated from different regions in China during 2016-2021. Immunoperoxidase monolayer assay showed that these viral strains shared strong cross-reaction with the previously reported caprine enterovirus CEV-JL14. Alignment analysis of the complete nucleotide sequences revealed 79.2%-87.8% and 75.0%-76.7% sequence identity of these novel caprine enterovirus strains to CEV-JL14 and TB4-OEV, respectively. Phylogenetic analyses clustered these novel strains to EV-G based on the amino acid sequences of P1 and 2C+3CD. Moreover, phylogenetic analysis of these caprine enterovirus strains identified three new EV-G types using VP1 sequences. These results demonstrate the genetic variations and the evolution of caprine enterovirus.

Keywords: CEV-JL14; alignment analysis; caprine/ovine enterovirus; evolution; genotype; phylogenetic analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Viral
  • China / epidemiology
  • Enterovirus Infections* / epidemiology
  • Enterovirus Infections* / genetics
  • Enterovirus Infections* / veterinary
  • Enterovirus* / genetics
  • Genome, Viral
  • Goats
  • Phylogeny
  • RNA, Viral / genetics
  • Sheep

Substances

  • Antigens, Viral
  • RNA, Viral

Grants and funding

This research was funded by the National Key Research and Development Program, grant numbers 2017YFD0500104 and 2016YFD0500904, and the National Natural Science Foundation of China, grant number 31572531.