Effects of a Biocomplex Formed by Two Scaffold Biomaterials, Hydroxyapatite/Tricalcium Phosphate Ceramic and Fibrin Biopolymer, with Photobiomodulation, on Bone Repair

Carlos Henrique Bertoni Reis; Rogerio Leone Buchaim; Karina Torres Pomini; Abdul Latif Hamzé; Isabella Vasconcelos Zattiti; Marco Antonio Hungaro Duarte; Murilo Priori Alcalde; Benedito Barraviera; Rui Seabra Ferreira Júnior; Fenelon Martinho Lima Pontes; Carlos Roberto Grandini; Adriana de Cássia Ortiz; Simone Ortiz Moura Fideles; Renata Maria de Camargo Eugênio; Geraldo Marco Rosa Junior; Daniel de Bortoli Teixeira; Eliana de Souza Bastos Mazuqueli Pereira; João Paulo Galletti Pilon; Maria Angelica Miglino; Daniela Vieira Buchaim

doi:10.3390/polym14102075

Effects of a Biocomplex Formed by Two Scaffold Biomaterials, Hydroxyapatite/Tricalcium Phosphate Ceramic and Fibrin Biopolymer, with Photobiomodulation, on Bone Repair

Polymers (Basel). 2022 May 19;14(10):2075. doi: 10.3390/polym14102075.

Authors

Carlos Henrique Bertoni Reis^{1

2}, Rogerio Leone Buchaim^{2

3}, Karina Torres Pomini^{2

4}, Abdul Latif Hamzé⁵, Isabella Vasconcelos Zattiti⁵, Marco Antonio Hungaro Duarte⁶, Murilo Priori Alcalde⁷, Benedito Barraviera^{8

9}, Rui Seabra Ferreira Júnior^{8

9}, Fenelon Martinho Lima Pontes¹⁰, Carlos Roberto Grandini¹¹, Adriana de Cássia Ortiz², Simone Ortiz Moura Fideles², Renata Maria de Camargo Eugênio⁵, Geraldo Marco Rosa Junior^{7

12}, Daniel de Bortoli Teixeira^{4

13}, Eliana de Souza Bastos Mazuqueli Pereira⁴, João Paulo Galletti Pilon^{1

14}, Maria Angelica Miglino³, Daniela Vieira Buchaim^{4

15}

Affiliations

¹ UNIMAR Beneficent Hospital (HBU), University of Marilia (UNIMAR), Marilia 17525-160, Brazil.
² Department of Biological Sciences, Bauru School of Dentistry (FOB/USP), University of São Paulo, Bauru 17012-901, Brazil.
³ Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), São Paulo 05508-270, Brazil.
⁴ Postgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marilia 17525-902, Brazil.
⁵ Medical School, University of Marilia (UNIMAR), Marilia 17525-160, Brazil.
⁶ Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo (FOB/USP), Bauru 17012-901, Brazil.
⁷ Department of Health Science, Unisagrado University Center, Bauru 17011-160, Brazil.
⁸ Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (Univ Estadual Paulista, UNESP), Botucatu 18610-307, Brazil.
⁹ Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP-Univ Estadual Paulista), Botucatu 18618-687, Brazil.
¹⁰ Chemistry Department, Faculty of Science, São Paulo State University (UNESP-Univ Estadual Paulista), Bauru 17033-360, Brazil.
¹¹ Laboratório de Anelasticidade e Biomateriais, Physics Department, Faculty of Science, São Paulo State University (UNESP-Univ Estadual Paulista), Bauru 17033-360, Brazil.
¹² Faculdade Ibero Americana de São Paulo, FIASP, Piraju 18810-818, Brazil.
¹³ Postgraduate Program in Animal Health, Production and Environment, University of Marilia (UNIMAR), Marília 17525-902, Brazil.
¹⁴ Postgraduate Program in Speech Therapy, Sao Paulo State University (UNESP-Univ Estadual Paulista), Marília 17525-900, Brazil.
¹⁵ Teaching and Research Coordination of the Medical School, University Center of Adamantina (UniFAI), Adamantina 17800-000, Brazil.

Abstract

There are several treatment methods available for bone repair, although the effectiveness becomes limited in cases of large defects. The objective of this pre-clinical protocol was to evaluate the grafting of hydroxyapatite/tricalcium phosphate (BCP) ceramic biomaterial (B; QualyBone BCP^®, QualyLive, Amadora, Portugal) together with the heterologous fibrin biopolymer (FB; CEVAP/UNESP Botucatu, Brazil) and with photobiomodulation (PBM; Laserpulse^®, Ibramed, Amparo, Brazil) in the repair process of bone defects. Fifty-six rats were randomly divided into four groups of seven animals each: the biomaterial group (G1/B), the biomaterial plus FB group (G2/BFB); the biomaterial plus PBM group (G3/B + PBM), and the biomaterial plus FB plus PBM group (G4/BFB + PBM). After anesthesia, a critical defect was performed in the center of the rats' parietal bones, then filled and treated according to their respective groups. The rats were euthanized at 14 and 42 postoperative days. Histomorphologically, at 42 days, the G4/BFB + PBM group showed a more advanced maturation transition, with more organized and mature bone areas forming concentric lamellae. A birefringence analysis of collagen fibers also showed a more advanced degree of maturation for the G4/BFB + PBM group. In the comparison between the groups, in the two experimental periods (14 and 42 days), in relation to the percentage of formation of new bone tissue, a significant difference was found between all groups (G1/B (5.42 ± 1.12; 21.49 ± 4.74), G2/BFB (5.00 ± 0.94; 21.77 ± 2.83), G3/B + PBM (12.65 ± 1.78; 29.29 ± 2.93), and G4/BFB + PBM (12.65 ± 2.32; 31.38 ± 2.89)). It was concluded that the use of PBM with low-level laser therapy (LLLT) positively interfered in the repair process of bone defects previously filled with the biocomplex formed by the heterologous fibrin biopolymer associated with the synthetic ceramic of hydroxyapatite and tricalcium phosphate.

Keywords: biomaterials; bone regeneration; bone repair; fibrin; fibrin tissue adhesive; low-level laser therapy; photobiomodulation.

Grants and funding

This research received no external funding.