Curcumin-Loaded Liposome Preparation in Ultrasound Environment under Pressurized Carbon Dioxide

Wahyudiono; Jiayang He; Xin Hu; Siti Machmudah; Keiji Yasuda; Seiichi Takami; Hideki Kanda; Motonobu Goto

doi:10.3390/foods11101469

Curcumin-Loaded Liposome Preparation in Ultrasound Environment under Pressurized Carbon Dioxide

Foods. 2022 May 18;11(10):1469. doi: 10.3390/foods11101469.

Authors

Wahyudiono^{1

2}, Jiayang He¹, Xin Hu¹, Siti Machmudah³, Keiji Yasuda⁴, Seiichi Takami¹, Hideki Kanda¹, Motonobu Goto^{1

5}

Affiliations

¹ Department of Materials Process Engineering, Nagoya University, Nagoya 464-8603, Japan.
² New Industry Creation Hatchery Center, Tohoku University, 6-6-10 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
³ Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, Surabaya 60111, Indonesia.
⁴ Department of Chemical Systems Engineering, Nagoya University, Nagoya 464-8603, Japan.
⁵ Super Critical Technology Centre Co., Ltd., Kuwana 511-0838, Japan.

Abstract

Curcumin-loaded liposomes were prepared using a supercritical carbon dioxide (SCCO2)−ultrasound environment system. The experiments were performed at temperatures of 40−70 °C and pressures of 10−25 MPa in a batch system with ultrasonication for 60 min. Transmission electron microscopy (TEM) images revealed liposome products with spherical morphologies and diameters of <100 nm. Dynamic light scattering (DLS) analysis indicated that the curcumin-loaded liposome nanosuspension exhibited good stability. Changing the operating conditions influenced the amount of liposome-encapsulated curcumin; as the operating temperature or pressure increased, the diameter of the liposome products and the amount of liposome-encapsulated curcumin increased and decreased, respectively. Herein, we described an innovative and practical organic-solvent-free method for generating liposomes from phospholipids.

Keywords: curcumin; encapsulation; liposome; phospholipid; supercritical.

Abstract

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