Chronic Supplementation of 2S-Hesperidin Improves Acid-Base Status and Decreases Lactate at FatMax, at Ventilatory Threshold 1 and 2 and after an Incremental Test in Amateur Cyclists

Francisco Javier Martínez-Noguera; Pedro E Alcaraz; Jorge Carlos-Vivas; Cristian Marín-Pagán

doi:10.3390/biology11050736

Chronic Supplementation of 2S-Hesperidin Improves Acid-Base Status and Decreases Lactate at FatMax, at Ventilatory Threshold 1 and 2 and after an Incremental Test in Amateur Cyclists

Biology (Basel). 2022 May 11;11(5):736. doi: 10.3390/biology11050736.

Authors

Francisco Javier Martínez-Noguera¹, Pedro E Alcaraz¹, Jorge Carlos-Vivas², Cristian Marín-Pagán¹

Affiliations

¹ Research Center for High Performance Sport, Catholic University of Murcia (UCAM), Campus de los Jerónimos Nº 135, 30107 Murcia, Spain.
² Health, Economy, Motricity and Education Research Group (HEME), Faculty of Sport Sciences, University of Extremadura, Avda. de Elvas, s/n., 06006 Badajoz, Spain.

Abstract

Chronic supplementation with 2S-hesperidin improves performance; however, the mechanisms underlying this effect have not yet been explored. Therefore, the aim of this study was to assess whether changes in acid-base status may be associated with improved performance after 2S-hesperidin supplementation compared to microcellulose (placebo). Forty amateur cyclists (n = 20 per group) underwent a rectangular test where capillary blood samples were taken at baseline, FatMax1, VT1, VT2, P_MAX, FatMax2 and EPOC to measure acid-base parameters. After eight weeks of 2S-hesperidin supplementation (500 mg/d) increased HCO₃^-, SBC, ABE (p ≤ 0.05) and decreased Lac were found at FatMax1, VT1, FatMax2 and EPOC (p ≤ 0.05), while decreased Lac at VT2 was found with a large effect size (ES = 1.15) compared to placebo. Significant group differences in the area under the curve were observed when comparing pre-post-intervention pH changes (p = 0.02) between groups. Chronic supplementation with 2S-hesperidin improved acid-base status and Lac, both at low-moderate and submaximal intensities, improving recovery after exercise-to-exhaustion in amateur cyclists.

Keywords: endurance; exercise; flavonoids; metabolism; performance; polyphenols; sport nutrition.

Grants and funding

The authors declare that this study has been financed by HTBA (Murcia, Spain), who kindly provided the product Cardiose^®, but they did not participate in the experimental design, data collection, data analysis, interpretation of the data, writing of the manuscript, or in the decision to publish the results.