Isolation of B-constituent through selective complex-induced precipitation of hordein with ι-carrageenan

Feifan Li; Kunlun Huang; Yunbo Luo; Xiaohong Mei

doi:10.1016/j.ijbiomac.2022.05.147

Isolation of B-constituent through selective complex-induced precipitation of hordein with ι-carrageenan

Int J Biol Macromol. 2022 Jul 1:212:508-516. doi: 10.1016/j.ijbiomac.2022.05.147. Epub 2022 May 24.

Authors

Feifan Li¹, Kunlun Huang¹, Yunbo Luo¹, Xiaohong Mei²

Affiliations

¹ College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, China; Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture and Rural Affairs of P.R., Beijing 100083, China.
² College of Food Science & Nutritional Engineering, China Agricultural University, Beijing, 100083, China; Center of Food Colloids and Delivery of Functionality, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: mxh@cau.edu.cn.

PMID: 35623460
DOI: 10.1016/j.ijbiomac.2022.05.147

Abstract

The complex behavior of the crude hordein with ι-carrageenan (Car) as a function of pH (11.0-3.0) and Hordein/Car mass ratios (20:1-1:1, w/w) was studied through zeta potential analysis, turbidimetric titration and SDS-PAGE. By preferential binding with Car, B-hordein was isolated from the crude hordein at pH_max (6.2) and Hordein/Car mass ratio of 15:1, which was further confirmed by LC-MS/MS analysis. The results of zeta potential and size of separated B-hordein and C-hordein suggested that the difference in charge density was the main driving force of selective complexation between hordein and Car. Simultaneously, Fourier transform infrared spectroscopy also confirmed the existence of strong electrostatic interaction between B-hordein and Car. Additionally, the more ordered secondary structure of B-hordein at pH_max might be beneficial to its preferential binding with Car. This study further promotes the application of B-hordein in food industry.

Keywords: B-hordein; Isolation; Selective complexation.

MeSH terms

Carrageenan / chemistry
Chromatography, Liquid
Glutens* / chemistry
Spectroscopy, Fourier Transform Infrared
Tandem Mass Spectrometry*

Substances

Glutens
Carrageenan