Metabolomic Mechanisms of Radix Fici Hirtae against Carbon Tetrachloride-Induced Acute Liver Damage in Mice

Fang-Yu Zhou; Ting Quan; Shi-Jian Xiang; Hui Li; Hui-Yuan Chen; Yu-Xuan Yang; Sha-Sha Li

doi:10.1155/2022/9157465

Metabolomic Mechanisms of Radix Fici Hirtae against Carbon Tetrachloride-Induced Acute Liver Damage in Mice

Evid Based Complement Alternat Med. 2022 May 17:2022:9157465. doi: 10.1155/2022/9157465. eCollection 2022.

Authors

Fang-Yu Zhou¹, Ting Quan¹, Shi-Jian Xiang², Hui Li³, Hui-Yuan Chen¹, Yu-Xuan Yang¹, Sha-Sha Li¹

Affiliations

¹ Department of Pharmacy, The First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
² Department of Pharmacy, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, Guangdong, China.
³ Department of Traditional Chinese Medicine, Guangzhou Red Cross Hospital, Medicine College, Jinan University, Guangzhou 510220, China.

Abstract

Background: Radix Fici Hirtae (RFH), known as Cantonese ginseng, is an alternative folk medicine that is widely used to treat various diseases in southern China. The aim of this study was to investigate the effect and metabolic mechanisms of pretreatment with RFH on the serum metabolic profiles of carbon tetrachloride (CCl₄) induced acute liver injury in mice.

Methods: Mice fed with the water extract of RFH at a dose of 1.5 g/kg and 0.75 g/kg for consecutive 7 days, and then serum samples were taken for the metabolomic analysis. Furthermore, the bioinformatics and pathways analysis were measured.

Results: The UHPLC-Orbitrap/MS based-metabolomic analysis identified 20 differential metabolic markers in serum of CCl₄-induced liver injury mice compared to that of the normal controls, which were mainly related to the metabolism of amino acids and fatty acids. Furthermore, most of these biomarkers contributing to CCl₄ induction were ameliorated by RFH, and the bioinformatics and pathways analysis revealed that therapeutic actions of RFH were mainly involved in the regulation of the oxidative stress responses and energy homeostasis.

Conclusion: These findings provide potential metabolic mechanism for future study and allow for hypothesis generation about the hepatoprotective effects of Radix Fici Hirtae.