A modified surgical procedure using minimally invasive ileocolic vein perfusion in a mouse intrahepatic islet transplant model

Lei Zhang; Ali Turan; Haval Shirwan; Esma S Yolcu

doi:10.1016/j.xpro.2022.101416

A modified surgical procedure using minimally invasive ileocolic vein perfusion in a mouse intrahepatic islet transplant model

STAR Protoc. 2022 May 19;3(2):101416. doi: 10.1016/j.xpro.2022.101416. eCollection 2022 Jun 17.

Authors

Lei Zhang¹, Ali Turan², Haval Shirwan^{1

2}, Esma S Yolcu^{1

2}

Affiliations

¹ Department of Child Health, University of Missouri, Columbia, MO 65211, USA.
² Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, MO 65211, USA.

Abstract

Murine intrahepatic islet transplantation is a clinically relevant but technically challenging surgical procedure because of frequent lethal postoperative bleeding. Here, we describe a protocol for mouse pancreatic islet isolation, purification, and culture. Besides, we also describe a protocol for intrahepatic islet transplantation through the ileocolic vein. Intrahepatic islet transplantation through the ileocolic vein, as opposed to traditional islet perfusion via the main portal vein, has the advantage of improving recovery after surgery and may facilitate islet survival and function in preclinical settings. For complete details on the use and execution of this protocol, please refer to Shrestha et al. (2020).

Keywords: Health Sciences; Model Organisms.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Disease Models, Animal
Islets of Langerhans Transplantation* / methods
Mesenteric Veins / surgery
Mice
Perfusion
Portal Vein / surgery
Transplants*

Abstract

Publication types

MeSH terms

Grants and funding