Protocol for characterizing strigolactones released by plant roots

Jian You Wang; Guan-Ting Erica Chen; Muhammad Jamil; Justine Braguy; Salim Sioud; Kit Xi Liew; Aparna Balakrishna; Salim Al-Babili

doi:10.1016/j.xpro.2022.101352

Protocol for characterizing strigolactones released by plant roots

STAR Protoc. 2022 May 18;3(2):101352. doi: 10.1016/j.xpro.2022.101352. eCollection 2022 Jun 17.

Authors

Jian You Wang^{1

2}, Guan-Ting Erica Chen^{1

2

3}, Muhammad Jamil^{1

2}, Justine Braguy^{1

2

3}, Salim Sioud⁴, Kit Xi Liew^{1

2}, Aparna Balakrishna^{1

2}, Salim Al-Babili^{1

2

3}

Affiliations

¹ The BioActives Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
² Center for Desert Agriculture, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
³ Plant Science Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
⁴ Analytical Chemistry Core Lab, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.

Abstract

The plant hormone strigolactones (SLs) are secreted by plant roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required for collection, processing, and analysis of plant root exudates using the C₁₈ column for SL extraction, followed by liquid chromatography-mass spectrometry (LC-MS) for SL quantification. We then describe image processing by SeedQuant, an open-source artificial-intelligence-based software, for measuring the biological activity of SLs in inducing root parasitic plant seed germination. For complete details on the use and execution of this protocol, please refer to Wang et al. (2019) and Braguy et al. (2021).

Keywords: Mass Spectrometry; Metabolism; Metabolomics; Model Organisms; Plant sciences.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, Liquid
Heterocyclic Compounds, 3-Ring / analysis
Lactones* / analysis
Plant Roots* / chemistry
Plants / chemistry

Substances

GR24 strigolactone
Heterocyclic Compounds, 3-Ring
Lactones