Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct

Bo Wang; Mickey Shah; Lakiesha N Williams; Amy L de Jongh Curry; Yi Hong; Ge Zhang; Jun Liao

doi:10.1007/978-1-0716-2261-2_4

Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct

Methods Mol Biol. 2022:2485:55-70. doi: 10.1007/978-1-0716-2261-2_4.

Authors

Bo Wang¹, Mickey Shah², Lakiesha N Williams³, Amy L de Jongh Curry⁴, Yi Hong⁵, Ge Zhang⁶, Jun Liao⁷

Affiliations

¹ Joint Department of Biomedical Engineering, Marquette University and the Medical College of Wisconsin, Milwaukee, WI, USA.
² Department of Biomedical Engineering, The University of Akron, Akron, OH, USA.
³ Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
⁴ Department of Biomedical Engineering, University of Memphis, Memphis, TN, USA.
⁵ Department of Bioengineering, University of Texas at Arlington, Arlington, TX, USA.
⁶ Department of Biomedical Engineering, The University of Akron, Akron, OH, USA. ge10@uakron.edu.
⁷ Department of Bioengineering, University of Texas at Arlington, Arlington, TX, USA. jun.liao@uta.edu.

Abstract

Cardiac tissue engineering/regeneration using decellularized myocardium has attracted great research attention due to its potential benefit to myocardial infarction (MI) treatment. Here, we described an optimal decellularization protocol to generate 3D porcine myocardial scaffolds with well-preserved cardiomyocyte lacunae, myocardial slices as a biomimetic cell culture and delivery platform, and a multi-stimulation bioreactor that is able to provide coordinated mechanical and electrical stimulations for facilitating cardiac construct development.

Keywords: Acellular myocardial scaffolds; Acellular myocardial slices; Bioreactor; Cardiac tissue engineering/regeneration; Decellularization; Electrical simulation; Mechanical simulation.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques
Myocardium
Myocytes, Cardiac
Swine
Tissue Engineering* / methods
Tissue Scaffolds*

Abstract

Publication types

MeSH terms

Grants and funding