Exogenous nitrogen from riverine exports promotes soil methane production in saltmarshes in China

Abstract

Methane emissions from saltmarshes can potentially promote climate warming. Soil methane production is positively correlated with methane emissions from saltmarshes. Understanding the factors influencing soil methane production will improve the prediction of methane emissions, but an investigation of these factors has not been conducted in saltmarshes in China. We collected soils from native Phragmites australis and invasive Spartina alterniflora saltmarshes along the coast of China; the soil potential methane production (PMP) was determined by incubation experiments. The large-scale investigation results showed that the ratios of methanogens relative to sulfate-reducing bacteria (RMRS) and total organic carbon (TOC) were positively correlated with soil PMP for both species. Dissolved inorganic nitrogen (DIN) was positively correlated with the soil PMP of P. australis saltmarshes, and plant biomass was positively correlated with the soil PMP of S. alterniflora saltmarshes. Our results showed that exogenous nitrogen from riverine exports was positively correlated with DIN and plant biomass in both P. australis and S. alterniflora saltmarshes. In addition, exogenous nitrogen was also positively correlated with TOC in S. alterniflora saltmarshes. Consequently, exogenous nitrogen indirectly promoted soil methane production in P. australis saltmarshes by increasing the DIN and promoted soil methane production in S. alterniflora saltmarshes by enhancing the TOC and plant biomass. Moreover, we found that the promoting effect of DIN on the soil PMP of P. australis saltmarshes increased when the incubation temperature increased from 15 °C to 25 °C. Thus, the promoting effect of exogenous nitrogen on the soil methane production in P. australis saltmarshes might be strengthened in the peak of growing season. Our findings are the first to confirm that exogenous nitrogen inputs from rivers indirectly promote soil methane production in P. australis and S. alterniflora saltmarshes and provide new insights into the factors responsible for soil methane production in saltmarshes.

Keywords: Biogeographical scale; Exogenous nitrogen; Methanogens; Saltmarsh; Soil methane production; Sulfate-reducing bacteria.