Insight into structural composition of dissolved organic matter in saline-alkali soil by fluorescence spectroscopy coupled with self-organizing map and structural equation modeling

Abstract

Soil salinization has been occurring all over the world, which severely affected crop production and threatened the life of mankind. It is necessary to take serious steps to improve soil fertility for the sustainability and productive capacity of agriculture. Soil samples of different depths were collected from native vegetation communities (Comm. Phragmites communis (CPC) and Comm. Populus alba (CPA)) and irrigated crops (corn fields (CFD) and seed melon fields (SMF)) in Hetao irrigation area of China. Three dimensional excitation-emission matrix (EEM) fluorescence technology combined with self-organizing map were used to analyze the dissolved organic matter (DOM) composition and structural characteristics in saline-alkali soils and its spatial distribution under different vegetation covers. Critical factors were recognized by classification and regression tree (CART) for distinguishing soil samples, and latent factors were revealed with structural equation modeling (SEM) for improving the humification degree of DOM from saline soils in Hetao irrigation area. Five components were obtained in the DOM substances, i.e., tyrosine-like (C1), tryptophan-like (C2), UV fulvic-like (C3), visible fulvic-like (C4) and humic-like (C5). The protein-like peaks were all obvious, and the fulvic-like peaks (600-735 a.u.) were conspicuous in the CPC soil than in others, except CFD1 and SMF1. C1 was the critical factor to distinguish native vegetation from irrigated crops, and C1 and C2 were the critical factors to distinguish CFD from SMF. Contrary to the HA/FA (0.20) and A/C (0.25), the path coefficient (-0.15) of sources with T/H was negative, indicating that the incremental contents of fluorenscense substances were in the sequences of protein-like > visible fulvic-like > UV fulvic-like > humic-like, affecting by the allochthonous. C1 (1.00) and C4 (1.00) were the primary components for improving the humification degree of DOM, which were principally originated from plant debris. EEM combined with self-organizing map, CART and SEM is an efficient way to distinguish different salinized soils and reveal the latent factors for improving the soil fertility.

Keywords: Classification and regression tree; Fluorescence spectroscopy; Self-organizing map; Soil dissolved organic matter; Structural equation modeling.