A hemicyanine-based near-infrared fluorescent probe for vapor-phase hydrazine detection and bioimaging in a complete aqueous media

Yang Wang; Xiao-Lei Xue; Qi Zhang; Kun-Peng Wang; Shaojin Chen; Linsheng Tang; Zhi-Qiang Hu

doi:10.1016/j.saa.2022.121406

A hemicyanine-based near-infrared fluorescent probe for vapor-phase hydrazine detection and bioimaging in a complete aqueous media

Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 15:279:121406. doi: 10.1016/j.saa.2022.121406. Epub 2022 May 18.

Authors

Yang Wang¹, Xiao-Lei Xue², Qi Zhang², Kun-Peng Wang³, Shaojin Chen², Linsheng Tang⁴, Zhi-Qiang Hu⁵

Affiliations

¹ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
² Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
³ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: wangkunpeng@qust.edu.cn.
⁴ College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
⁵ Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. Electronic address: huzhiqiang@qust.edu.cn.

PMID: 35617838
DOI: 10.1016/j.saa.2022.121406

Abstract

A novel near-infrared fluorescent probe CyOE based on hemicyanine dye containing acetyl as a recognition site is reported. The probe CyOE shows high selectivity and sensitivity (LOD = 82 nM, 2.58 ppb), as well as good water solubility and quantitative detectability of hydrazine in the concentration range of 0-75 μM (R² = 0.993). Moreover, CyOE has a significant increase in fluorescence at 735 nm with the addition of N₂H₄, which provides a rapid, colorimetric and gas-phase detection method for N₂H₄ in both aqueous solution and real water samples. In addition, CyOE is successfully utilized to visualize hydrazine in cells with low cytotoxicity and high cell permeability.

Keywords: Bioimaging; Fluorescent probe; Hemicyanine; Hydrazine; Near-infrared fluorescence.

MeSH terms

Carbocyanines
Fluorescent Dyes* / toxicity
Gases
HeLa Cells
Humans
Hydrazines*
Spectrometry, Fluorescence / methods
Water

Substances

Carbocyanines
Fluorescent Dyes
Gases
Hydrazines
tetramethylene hemicyanine
Water