Intergenerational and biological effects of roxithromycin and polystyrene microplastics to Daphnia magna

Jiaqiang Liu; Haohan Yang; Qingjun Meng; Qiyan Feng; Zhenhua Yan; Jianchao Liu; Zhigang Liu; Zhengxie Zhou

doi:10.1016/j.aquatox.2022.106192

Intergenerational and biological effects of roxithromycin and polystyrene microplastics to Daphnia magna

Aquat Toxicol. 2022 Jul:248:106192. doi: 10.1016/j.aquatox.2022.106192. Epub 2022 May 7.

Authors

Jiaqiang Liu¹, Haohan Yang², Qingjun Meng¹, Qiyan Feng¹, Zhenhua Yan³, Jianchao Liu³, Zhigang Liu⁴, Zhengxie Zhou⁴

Affiliations

¹ School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China.
² School of Environment Science and Spatial Informatics, China University of Mining and Technology, Xuzhou, Jiangsu 221116, China; School of Environmental Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127, China. Electronic address: 6107@cumt.edu.cn.
³ Key Laboratory for Integrated Regulation and Resources Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China.
⁴ Ningbo Water Supply Co Ltd, Ningbo 315041, China.

PMID: 35617774
DOI: 10.1016/j.aquatox.2022.106192

Abstract

The influence of microplastics (MPs) on transgenerational effects of pharmaceuticals are drawing growing attention, however, whether aged process will alter the carrier effects of MPs were unknown. In this study, the intergenerational toxicity of single and combined exposure of polystyrene microplastics (PS-MPs) and roxithromycin (ROX) were investigated at the environmentally related concentrations, using Daphina magna as test organism. In the presence of UV-aged PS-MPs, the survival of D. magna for maternal generation (F0) at ROX concentration of 0.1 and 10 µg/L were increased by 20% and 40%, respectively. Meanwhile, the inhibition effects of ROX on the number of offspring and intrinsic rate of natural increase were obviously moderated. All these reproductive toxicity of ROX and PS-MPs in the first offspring (F1) were further aggravated both for the single and combined exposure. And the adverse effects disappeared much easier for the single exposure compared to the co-exposure through subsequent recovery. The combined exposure resulted in the change of inhibition of ROX on the swimming velocity and acceleration of D. magna into induction, while the feeding behavior kept inhibited. The AChE activity was distinctly increased by 1.61-3.25 times for the single and combined treatments, and the induction level of UV-aged MPs was higher than that of original MPs. Oxidative stress of the single exposure of ROX and original PS-MPs was observed with obvious induction of T-AOC and SOD activity, while the significant increase of MDA content was observed for the co-exposure. Among all indicators, the biochemical biomarkers and time of first brood were attributed to a class among all indicators, indicating that the time of first brood might be the most sensitive reproductive toxicity index. These results illustrated that both maternal impacts and offspring quality need to be considered for assessment of interaction of emerging contaminants.

Keywords: Daphnia magna; Intergenerational toxicity; Roxithromycin; UV-aged polystyrene microplastics.

MeSH terms

Animals
Daphnia
Microplastics
Plastics
Polystyrenes / toxicity
Roxithromycin* / toxicity
Water Pollutants, Chemical* / toxicity

Substances

Microplastics
Plastics
Polystyrenes
Water Pollutants, Chemical
Roxithromycin